当代医学
噹代醫學
당대의학
CHINA CONTEMPORARY MEDICINE
2013年
31期
13-15
,共3页
杨静%王登奎%吴刚%庞东卫%程向晖
楊靜%王登奎%吳剛%龐東衛%程嚮暉
양정%왕등규%오강%방동위%정향휘
二阶导数极谱法%对氨基苯磺酸%盐酸萘乙二胺%血清%亚硝酸根(NO 2-)
二階導數極譜法%對氨基苯磺痠%鹽痠萘乙二胺%血清%亞硝痠根(NO 2-)
이계도수겁보법%대안기분광산%염산내을이알%혈청%아초산근(NO 2-)
Secondary derivative oscillographic polarography%Sulfanilic acid%N-(1-naphthyl) ethylenediamine dihydrochloride%Serum%Nitrite
目的：评价二阶导数示波极谱法测定人血清中NO 2-含量的方法。方法采用对氨基苯磺酸-盐酸萘乙二胺为测定体系，使用二阶导数极谱法测定血清中的NO 2-含量。结果该方法测得正常成人的血清NO 2-浓度为(2.33±0.06)mol/L，检出限为0.98 mmol/L，线性范围为0.98～46.38 mmol/L，精密度为5.0%，平均回收率为105.5%。结论该方法是一种简单，易操作的测定血清NO 2-的方法。
目的：評價二階導數示波極譜法測定人血清中NO 2-含量的方法。方法採用對氨基苯磺痠-鹽痠萘乙二胺為測定體繫，使用二階導數極譜法測定血清中的NO 2-含量。結果該方法測得正常成人的血清NO 2-濃度為(2.33±0.06)mol/L，檢齣限為0.98 mmol/L，線性範圍為0.98～46.38 mmol/L，精密度為5.0%，平均迴收率為105.5%。結論該方法是一種簡單，易操作的測定血清NO 2-的方法。
목적：평개이계도수시파겁보법측정인혈청중NO 2-함량적방법。방법채용대안기분광산-염산내을이알위측정체계，사용이계도수겁보법측정혈청중적NO 2-함량。결과해방법측득정상성인적혈청NO 2-농도위(2.33±0.06)mol/L，검출한위0.98 mmol/L，선성범위위0.98～46.38 mmol/L，정밀도위5.0%，평균회수솔위105.5%。결론해방법시일충간단，역조작적측정혈청NO 2-적방법。
Objective To evaluate the method of mensurating the content of NO 2-in human serum by secondary derivative oscillographic polarography. Methods Mensurate the content of NO 2- in human serum by secondary derivative oscillographic polarography in the system of sulfanilic acid and N-(1-naphthyl) ethylenediamine dihydrochloride. Results Normal adult serum NO 2-concentration is (2.33±0.06)mol/L,The sensitivity of the method was 0.98 mmol/L,the linear range was from 0.98 to 46.38 mmol/L,the precision was 5.0%,the analytical average recovery rate was 105.5%. Conclusion The method is a simple method and easy to operate.
