中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2014年
8期
1134-1137
,共4页
胃肿瘤%MAGE基因%外周血单核细胞%预后
胃腫瘤%MAGE基因%外週血單覈細胞%預後
위종류%MAGE기인%외주혈단핵세포%예후
Gastric neoplasms%Melanoma antigen gene%Peripheral blood mononuclear cells%Prognosis
目的 检测胃癌患者外周血中黑色素瘤抗原A1(MAGEA1)和黑色素瘤抗原A3(MAGEA3)基因表达,结合患者术后随访情况,分析MAGE基因表达与胃癌转移及预后的关系.方法 术前采集40例胃癌患者及20例健康人外周血,用RT-PCR方法检测外周血单核细胞(PBMC)中MAGEA1和MAGEA3基因mRNA.用RT-PCR方法检测胃癌组织中上述MAGE基因的表达,同时检测上述标本CEA表达.结果 47.5％(19/40)和25.0％(10/40)胃癌患者外周血PBMC中分别检测到MAGEA1和MAGEA3基因mRNA,而相应的胃癌组织中MAGEA1和MAGEA3基因表达率分别为60.0％ (24/40)和30.0％(12/40),相应的癌旁胃组织均不表达,其中有4例外周血两者均表达.在62.5％ (25/40)的胃癌患者外周血单核细胞(PBMC)中至少检测到一种MAGE基因mRNA;上述患者外周血PBMC中CEA基因mRNA的检出率为65.0％ (26/40),20例非肿瘤疾病患者的PBMC中均未检出MAGE基因mRNA.胃癌患者PBMC中MAGE基因mRNA的检出率与肿瘤淋巴结转移、TNM分期相关,而与肿瘤分化程度、浸润深度、肿瘤大小、远处转移无关.结论 胃癌患者外周血MAGE基因表达与肿瘤淋巴结转移、TNM分期密切相关,表达阳性的患者预后差.
目的 檢測胃癌患者外週血中黑色素瘤抗原A1(MAGEA1)和黑色素瘤抗原A3(MAGEA3)基因錶達,結閤患者術後隨訪情況,分析MAGE基因錶達與胃癌轉移及預後的關繫.方法 術前採集40例胃癌患者及20例健康人外週血,用RT-PCR方法檢測外週血單覈細胞(PBMC)中MAGEA1和MAGEA3基因mRNA.用RT-PCR方法檢測胃癌組織中上述MAGE基因的錶達,同時檢測上述標本CEA錶達.結果 47.5％(19/40)和25.0％(10/40)胃癌患者外週血PBMC中分彆檢測到MAGEA1和MAGEA3基因mRNA,而相應的胃癌組織中MAGEA1和MAGEA3基因錶達率分彆為60.0％ (24/40)和30.0％(12/40),相應的癌徬胃組織均不錶達,其中有4例外週血兩者均錶達.在62.5％ (25/40)的胃癌患者外週血單覈細胞(PBMC)中至少檢測到一種MAGE基因mRNA;上述患者外週血PBMC中CEA基因mRNA的檢齣率為65.0％ (26/40),20例非腫瘤疾病患者的PBMC中均未檢齣MAGE基因mRNA.胃癌患者PBMC中MAGE基因mRNA的檢齣率與腫瘤淋巴結轉移、TNM分期相關,而與腫瘤分化程度、浸潤深度、腫瘤大小、遠處轉移無關.結論 胃癌患者外週血MAGE基因錶達與腫瘤淋巴結轉移、TNM分期密切相關,錶達暘性的患者預後差.
목적 검측위암환자외주혈중흑색소류항원A1(MAGEA1)화흑색소류항원A3(MAGEA3)기인표체,결합환자술후수방정황,분석MAGE기인표체여위암전이급예후적관계.방법 술전채집40례위암환자급20례건강인외주혈,용RT-PCR방법검측외주혈단핵세포(PBMC)중MAGEA1화MAGEA3기인mRNA.용RT-PCR방법검측위암조직중상술MAGE기인적표체,동시검측상술표본CEA표체.결과 47.5％(19/40)화25.0％(10/40)위암환자외주혈PBMC중분별검측도MAGEA1화MAGEA3기인mRNA,이상응적위암조직중MAGEA1화MAGEA3기인표체솔분별위60.0％ (24/40)화30.0％(12/40),상응적암방위조직균불표체,기중유4예외주혈량자균표체.재62.5％ (25/40)적위암환자외주혈단핵세포(PBMC)중지소검측도일충MAGE기인mRNA;상술환자외주혈PBMC중CEA기인mRNA적검출솔위65.0％ (26/40),20례비종류질병환자적PBMC중균미검출MAGE기인mRNA.위암환자PBMC중MAGE기인mRNA적검출솔여종류림파결전이、TNM분기상관,이여종류분화정도、침윤심도、종류대소、원처전이무관.결론 위암환자외주혈MAGE기인표체여종류림파결전이、TNM분기밀절상관,표체양성적환자예후차.
Objective In the detection of peripheral blood in patients with gastric cancer the melanoma antigen A1 (MAGEA1) and the melanoma antigen A3 (MAGEA3) gene expression,combined with the conditions of patients with postoperative follow-up analysis MAGE gene expression and the relationship between gastric cancer metastasis and prognosis.Methods Peripheral blood was obtained from 40 patients with gastric carcinoma and 20 patients with benign diseases.The mRNA of the MAGEA1 and MAGEA3 genes in the peripheral blood mononuclear cells(PBMC) was detected by RT-PCR,meanwhile the MAGEA1 and MAGEA3 transcripts in gastric carcinoma tissues and corresponding nomal tissue were detected.In contrast with CEA gene,we detected its expression by nested RT-PCR.Results Of the 40 gastric carcinoma patients,MAGEA1 and MAGEA3 mRNA were positive in 47.5％ (17/40) and 25.0％ (10/40) of PBMC,respectively,and in 65.0％ (26/40) and 30.0％ (12/40) of gastric carcinoma tissues,the corresponding paracancerous gastric tissues were not expressed,of which 4 cases of peripheral blood both expression.In the PBMC of 40 gastric carcinoma patients,62.5％ (25/40) samples were detected to express at least one type of MAGE mRNA,the detection of CEA gene mRNA in peripheral blood of the patients in PBMCwas 65.0％ (26/40),20 patients with non neoplastic diseases of PBMC were not detected in the MAGE gene mRNA.There were 22.5％ (9/40) of 40 gastric carcinoma patients with CEA gene expression in PBMC.The detection rate of the MAGE gene of mRNA PBMC in patients with gastric cancer was not related with the lymph node metastasis,TNM stage,and the degree of tumor differentiation,depth of invasion,tumor size,distant metastasis.Conclusion Gastric cancer patients with peripheral blood MAGE gene expression is closely related to the tumor lymph node metastasis,TNM staging,express positive patients with poor prognosis.
