CAJ | 학술논문

通过PCR和RT-PCR在DNA和RNA水平上筛选鉴定SYTA基因T-DAN插入失活纯合突变体，当对野生型与突变体种子进行45℃/4h与50℃/1h 热激后，野生型种子萌发率分别达到100%与86%，而突变体种子萌发率分别只有83%与27%。当对野生型与突变体植株幼苗进行热激处理后，其存活率与叶片萎黄率相差不大，说明SYTA基因能显著提高拟南芥种子的耐热性，但拟南芥幼苗的耐热性不受SYTA基因的影响。
통과PCR화RT-PCR재DNA화RNA수평상사선감정SYTA기인T-DAN삽입실활순합돌변체，당대야생형여돌변체충자진행45℃/4h여50℃/1h 열격후，야생형충자맹발솔분별체도100%여86%，이돌변체충자맹발솔분별지유83%여27%。당대야생형여돌변체식주유묘진행열격처리후，기존활솔여협편위황솔상차불대，설명SYTA기인능현저제고의남개충자적내열성，단의남개유묘적내열성불수SYTA기인적영향。
One homozygous T-DNA mutant was identified at DNA and RNA levels by PCR and RT-PCR.After wild-type and mutant seeds were subjected to a heat shock treatment of 45℃/4h and 50℃/1h,wild-type seed germination rate reached 100% and 86%,mutant seed germination rates were only 83% and 27%.When wild-type and mutant seedlings were subjected to a heat shock treatment,their survival rate and leaf chlorosis rate were almost the same.The phenotype of the mutant and wild type after subj ected to heat shock shows that SYTA gene remarkably enhance the Arabidopsis seeds’ther-motolerance.But syta mutant seedlings don’t exhibit reduced basal thermotolerance and acquired ther-motolerance.