CAJ | 학술논문

目的：观察胃转流术对2型糖尿病大鼠肾脏氧化应激及蛋白激酶C（PKC）活性的影响。方法40只SD大鼠随机分为正常对照组（8只），糖尿病模型组（32只），模型组注射STZ（35 mg/kg），血糖稳定后确定成模30只，再将其分为糖尿病对照组（8只），假手术组（8只），手术组（14只）。术后8周检测各组大鼠空腹血糖、肾重指数（肾重/体重）、尿素氮（BUN）、血肌酐（Cr），测定各组大鼠肾组织总超氧化物歧化酶（TSOD）、谷胱甘肽过氧化酶（GSH-PX）活性，脂质过氧化代谢产物丙二醛（MDA）含量及PKC的活性，HE染色观察肾组织病理变化。结果与糖尿病对照组和假手术组相比，手术组能明显降低空腹血糖、改善肾重指数、BUN、Cr等指标（P＜0.05），同时肾组织抗氧化酶TSOD、GSH-PX活性增强，MDA含量减少，肾脏PKC活性降低（P＜0.05）。HE染色显示手术组病变也明显轻于糖尿病对照组和假手术组。结论胃转流术可能通过抑制2型糖尿病大鼠肾脏氧化应激及PKC活性增强发挥肾脏保护作用。
목적：관찰위전류술대2형당뇨병대서신장양화응격급단백격매C（PKC）활성적영향。방법40지SD대서수궤분위정상대조조（8지），당뇨병모형조（32지），모형조주사STZ（35 mg/kg），혈당은정후학정성모30지，재장기분위당뇨병대조조（8지），가수술조（8지），수술조（14지）。술후8주검측각조대서공복혈당、신중지수（신중/체중）、뇨소담（BUN）、혈기항（Cr），측정각조대서신조직총초양화물기화매（TSOD）、곡광감태과양화매（GSH-PX）활성，지질과양화대사산물병이철（MDA）함량급PKC적활성，HE염색관찰신조직병리변화。결과여당뇨병대조조화가수술조상비，수술조능명현강저공복혈당、개선신중지수、BUN、Cr등지표（P＜0.05），동시신조직항양화매TSOD、GSH-PX활성증강，MDA함량감소，신장PKC활성강저（P＜0.05）。HE염색현시수술조병변야명현경우당뇨병대조조화가수술조。결론위전류술가능통과억제2형당뇨병대서신장양화응격급PKC활성증강발휘신장보호작용。
Objective To observe the influence of gastric bypass on renal oxidative stress and the activity of protein kinase C in type 2 diabetic rats.Methods Forty SD rats were randomly divided into normal control group (n =8 ),diabetes model group(n=32),the diabetes model group were injected STZ(35mg/kg),when the blood glucose steady,there were 30 rats were considered as the successful diabetes model.Then they were randomly divided into diabetes control group(n=8),dia-betes sham operation group(n=8)and diabetes operation group(n=14).the kidney hypertrophy index(kidney weight/body weight,KW/BW),fasting blood glucose(FBG),blood urea nitrogen(BUN),serum creatinine(Cr),the activities of to-tal superoxide dismutase(TSOD),glutathione peroxidase(GSH-PX)and protein kinase C(PKC),content of malondialde-hyde(MDA)in renal tissue were measured after operation for 8 weeks.In addition,the renal tissue was observed by light mi-croscopy.Results In diabetes operation group,kidney hypertrophy index,FBG,BUN,Cr,content of MDA and the activity of PKC markedly decreased(P<0.05),the activities of TSOD and GSH-PX increased(P<0.05)compared with diabetes con-trol group and diabetes sham operation group.Renal pathologic changes in diabetes operation group were also improved. Conclusion The gastric bypass could protect the kidney of type 2 diabetic rats,which may be ascribed to the inhibitory effect of oxidative stress and the activity of PKC in renal tissue.