CAJ | 학술논문

试验研究在现代封闭循环海水工厂化养殖条件下，探寻抗肠炎功能蛋白添加剂新产品对石斑鱼生长、消化以及水质等的效应。在基础饲粮中分别添加0‰、1‰、2‰、3‰（0~3#组）的抗肠炎蛋白粉，分别饲喂初始体重为（64.80±0.56）g的4组点带石斑鱼640尾，每处理组4重复，正式试验期105 d。试验结果表明：①试验发现，饲粮添加2‰以上抗肠炎蛋白粉后，显著降低试验鱼应激反应副作用，即显著减缓采食量下降幅度，这是抗肠炎蛋白粉提高生长性能的原因所在，对鱼类养殖生产意义重大。②基础饲料中添加1‰~3‰的抗肠炎蛋白粉，明显提高点带石斑鱼的生长性能，增重率比对照组提高7.8%~13.9%，饲料系数比对照组降低9.4%~12.0%（P<0.05）。随抗肠炎蛋白粉添加量增加，水中氨氮含量出现递增特征。③抗肠炎蛋白粉对蛋白质和淀粉消化酶活力产生了显著影响，而对脂肪酶活力作用不明显。肝胰脏蛋白酶活力显著或极显著提高14.4%~56.4%。添加1‰和2‰抗肠炎蛋白粉，胃肠淀粉酶活力分别提高了24.1%、10.9%，肝胰脏淀粉酶活力分别提高7.6%、10.9%（P<0.05）。研究证明，工业化养殖点带石斑鱼幼鱼膨化颗粒饲料中，抗肠炎蛋白粉的适宜添加量为2‰~3‰。
시험연구재현대봉폐순배해수공엄화양식조건하，탐심항장염공능단백첨가제신산품대석반어생장、소화이급수질등적효응。재기출사량중분별첨가0‰、1‰、2‰、3‰（0~3#조）적항장염단백분，분별사위초시체중위（64.80±0.56）g적4조점대석반어640미，매처리조4중복，정식시험기105 d。시험결과표명：①시험발현，사량첨가2‰이상항장염단백분후，현저강저시험어응격반응부작용，즉현저감완채식량하강폭도，저시항장염단백분제고생장성능적원인소재，대어류양식생산의의중대。②기출사료중첨가1‰~3‰적항장염단백분，명현제고점대석반어적생장성능，증중솔비대조조제고7.8%~13.9%，사료계수비대조조강저9.4%~12.0%（P<0.05）。수항장염단백분첨가량증가，수중안담함량출현체증특정。③항장염단백분대단백질화정분소화매활력산생료현저영향，이대지방매활력작용불명현。간이장단백매활력현저혹겁현저제고14.4%~56.4%。첨가1‰화2‰항장염단백분，위장정분매활력분별제고료24.1%、10.9%，간이장정분매활력분별제고7.6%、10.9%（P<0.05）。연구증명，공업화양식점대석반어유어팽화과립사료중，항장염단백분적괄의첨가량위2‰~3‰。
This experiment aimed at investigating the effect of a new feed additive, anti-enteritis protein determined on growth performance, digestive enzyme activity and water quality of Epi-nephelus coioides in the industrial semi-recirculating culture condition. 640 trial fish [with aver-age initial weight of (64.80±0.56) g] were randomly allocated into 16 quadruplicated treatments, with basal feed adding four anti-enteritis protein levels of 0‰, 1‰, 2‰, 3‰(0~3# groups). Af-ter 105 days of cultivation, the results indicate that: ① 2#~3# significantly slowed down the de-scending range of feed intake caused by stress response. That is the essence of why anti-enteri-tis protein can improve growth performance and it is very important for fish farming. ②Weight gain ratio and feed coefficient in 1#~3# were 7.8%~13.9% and 9.4%~12.0% high-er than 0# (P<0.05). The ammonia nitrogen in aquatic water increases with the amount of additives. ③Anti-enteritis protein has obvi-ous significance on protease and amylase ac-tivity but the lipase activity. Liver-pancreas protease in 1#~3# dramatically improved 14.4%~56.4%. In 1#~2#, the stomach and intestine amylase rose 24.1%, 10.9%, and the liver-pancreas amylase rose 7.6%, 10.9%(P<0.05). In conclusion, the suitable addition amount is 2‰~3‰.