CAJ | 학술논문

随着春保护地西瓜种植面积的扩大和工厂化育苗的发展，西瓜苗期病害也越来越严重，有些常见于生长期的病害，现在在苗期也有较重发生。对湖北省宜城市流水镇育苗场采集的2份西瓜苗期病样，进行分离、纯化并回接病原菌，然后利用PCR扩增真菌保守的ITS序列，初步确定所采集病样1-a的致病菌为枯萎病病原菌[Fusarium oxysporum Schl. f. sp. niveum （E. F. Smith） Snyber et Hansen]，病样1-b 的致病菌为叶枯病病原菌[Alternaria cucumerina （Ell. et Ev.） Elliott]。本研究利用分子鉴定手段，避免了传统分类方法的繁琐、耗时和结果不稳定性等缺点。
수착춘보호지서과충식면적적확대화공엄화육묘적발전，서과묘기병해야월래월엄중，유사상견우생장기적병해，현재재묘기야유교중발생。대호북성의성시류수진육묘장채집적2빈서과묘기병양，진행분리、순화병회접병원균，연후이용PCR확증진균보수적ITS서렬，초보학정소채집병양1-a적치병균위고위병병원균[Fusarium oxysporum Schl. f. sp. niveum （E. F. Smith） Snyber et Hansen]，병양1-b 적치병균위협고병병원균[Alternaria cucumerina （Ell. et Ev.） Elliott]。본연구이용분자감정수단，피면료전통분류방법적번쇄、모시화결과불은정성등결점。
With the expansion of planting area and the development of the industrial watermelon seedling production in protected fields, the seedling diseases were becoming more and more seriously, and some common diseases of growing period occurred badly at the seedling stage. In the paper, firstly, we collected two samples with seedling diseases, and separated, purified and inoculated the pathogenic fungi. Secondly, we amplified the conserved ITS sequences of the fungi. Thirdly, we preliminary determinated the pathogenic fungus of sample 1-a and sample 1-b was Fusarium wilt [Fusarium oxysporum Schl. f. sp. niveum (E. F. Smith) Snyber et Hansen] and leaf blight [Alternaria cucumerina (Ell. et Ev.) Elliott] respectively. In the paper, we used molecular identification method, effectively avoiding the application of tedious, time-consuming and unstable traditional classification methods.