河北医药
河北醫藥
하북의약
HEBEI MEDICAL JOURNAL
2013年
18期
2731-2734
,共4页
三七皂甙%肝纤维化%线粒体
三七皂甙%肝纖維化%線粒體
삼칠조대%간섬유화%선립체
panax notoginseng saponins%hepatic fibrosis%mitochondria
目的探讨三七皂甙Rg1、Rb1对肝纤维化大鼠线粒体的保护作用及机制。方法将大鼠随机分为4组，分别为对照组、模型组、Rg1、Rb1保护组，用5％CCl4橄榄油胃饲复制大鼠肝纤维化模型，用透射电镜和光镜观察肝细胞形态学变化。用放射免疫法检测Ⅲ型前胶原（ procollagen type Ⅲ，PCⅢ）的水平。用[3H]油酸标记的生物膜法测定肝线粒体分泌型磷脂酶A2（secretory phospholipase A2，sPLA2）活性，用分光光度法测定肝线粒体MDA含量，SOD活性，Ca2＋-ATP酶，总ATP酶活性，用荧光偏振法测定肝线粒体膜的流动性，紫外分光光度法测定呼吸链复合体Ⅰ，Ⅱ，Ⅲ，Ⅳ的活性。结果组织学用电镜检测可见模型组细胞有粗大束状的胶原纤维沉淀。 HE染色和Masson胶原染色切片统计分析得出Rg1、Rb1组的纤维化程度与对照组比较有统计学意义（ P ＜0噜．01）。血清学检查显示Rg1、Rb1组显著抑制血清中ALT、PCⅢ的升高，提高线粒体SOD活性，降低MDA含量，抑制sPLA2的升高，提高线粒体膜Ca2＋-ATP酶活性，总ATP酶活性（ P ＜0．01）；生物物理学证实Rg1、Rb1组增加线粒体膜的流动性，降低膜的微粘度，显著增加呼吸链复合体Ⅲ，Ⅳ的活性（ P ＜0．01）。结论三七皂甙Rg1、Rb1通过对肝纤维化大鼠线粒体显著的保护机理而起到抗肝纤维化的作用。
目的探討三七皂甙Rg1、Rb1對肝纖維化大鼠線粒體的保護作用及機製。方法將大鼠隨機分為4組，分彆為對照組、模型組、Rg1、Rb1保護組，用5％CCl4橄欖油胃飼複製大鼠肝纖維化模型，用透射電鏡和光鏡觀察肝細胞形態學變化。用放射免疫法檢測Ⅲ型前膠原（ procollagen type Ⅲ，PCⅢ）的水平。用[3H]油痠標記的生物膜法測定肝線粒體分泌型燐脂酶A2（secretory phospholipase A2，sPLA2）活性，用分光光度法測定肝線粒體MDA含量，SOD活性，Ca2＋-ATP酶，總ATP酶活性，用熒光偏振法測定肝線粒體膜的流動性，紫外分光光度法測定呼吸鏈複閤體Ⅰ，Ⅱ，Ⅲ，Ⅳ的活性。結果組織學用電鏡檢測可見模型組細胞有粗大束狀的膠原纖維沉澱。 HE染色和Masson膠原染色切片統計分析得齣Rg1、Rb1組的纖維化程度與對照組比較有統計學意義（ P ＜0嚕．01）。血清學檢查顯示Rg1、Rb1組顯著抑製血清中ALT、PCⅢ的升高，提高線粒體SOD活性，降低MDA含量，抑製sPLA2的升高，提高線粒體膜Ca2＋-ATP酶活性，總ATP酶活性（ P ＜0．01）；生物物理學證實Rg1、Rb1組增加線粒體膜的流動性，降低膜的微粘度，顯著增加呼吸鏈複閤體Ⅲ，Ⅳ的活性（ P ＜0．01）。結論三七皂甙Rg1、Rb1通過對肝纖維化大鼠線粒體顯著的保護機理而起到抗肝纖維化的作用。
목적탐토삼칠조대Rg1、Rb1대간섬유화대서선립체적보호작용급궤제。방법장대서수궤분위4조，분별위대조조、모형조、Rg1、Rb1보호조，용5％CCl4감람유위사복제대서간섬유화모형，용투사전경화광경관찰간세포형태학변화。용방사면역법검측Ⅲ형전효원（ procollagen type Ⅲ，PCⅢ）적수평。용[3H]유산표기적생물막법측정간선립체분비형린지매A2（secretory phospholipase A2，sPLA2）활성，용분광광도법측정간선립체MDA함량，SOD활성，Ca2＋-ATP매，총ATP매활성，용형광편진법측정간선립체막적류동성，자외분광광도법측정호흡련복합체Ⅰ，Ⅱ，Ⅲ，Ⅳ적활성。결과조직학용전경검측가견모형조세포유조대속상적효원섬유침정。 HE염색화Masson효원염색절편통계분석득출Rg1、Rb1조적섬유화정도여대조조비교유통계학의의（ P ＜0로．01）。혈청학검사현시Rg1、Rb1조현저억제혈청중ALT、PCⅢ적승고，제고선립체SOD활성，강저MDA함량，억제sPLA2적승고，제고선립체막Ca2＋-ATP매활성，총ATP매활성（ P ＜0．01）；생물물이학증실Rg1、Rb1조증가선립체막적류동성，강저막적미점도，현저증가호흡련복합체Ⅲ，Ⅳ적활성（ P ＜0．01）。결론삼칠조대Rg1、Rb1통과대간섬유화대서선립체현저적보호궤리이기도항간섬유화적작용。
Objective To investigate the protective effect panax notoginseng saponins -Rg1, Rb1 on mitochondria of rats with hepatic fibrosis .Methods The rats were randomly divided into four groups , control group,model group,Rg1 group and Rb1group.The rat models with hepatic fibrosis were established by giving 5%CCI4 solution via gavage , then hepatic morphological changes were observed by transmission electron microscope and light microscope;procollagen type Ⅲ ( PCⅢ) levels were detected by radio-immuno assay;secretory phospholipase A2 (sPLA2) activity was tested by labeled E.coli membrane with [3H] oleic acid.The changes of mitochondrial membrane fluidity were detected by fluorescent polarization;MDA content,activities of SOD,Ca2+-ATPase and total ATPase were determined by spectrophotography;activities of respiratory chain complex Ⅰ,Ⅱ,Ⅲ,Ⅳ were detected by ultraviolet spectrophotometry .Results Transmission electron microscope showed that collagen fibrils and rough bunch of collagen fibrils precipitation were found in control group .The results of HE staining and Masson collagen staining slices demonstrated that there were significant differences in fibration degrees between Rg1,Rb1 groups and control group ( P <0.01).Serological testing showed that Rg 1 and Rb1 remarkably reduced the levels of ALT ,PCⅢin serum,enhanced SOD activity and decreased MDA levels .Rg 1 and Rb1 also significantly increased the activities of respiratory chain complex Ⅰ,Ⅲ,Ⅳ and total ATPase ( P <0.01). Conclusion Panax notoginseng saponins-Rg1,Rb1 has inhibitory effect on hepatic fibrosis through protecting the mitochondria of rats with liver fibrosis .
