CAJ | 학술논문

目的观察SHG-44神经胶质瘤干细胞分化为胶质瘤细胞前、后miR-92b基因表达的变化。方法从人胶质瘤SHG-44细胞中筛选出肿瘤干细胞并进行荧光免疫鉴定；将肿瘤干细胞诱导分化为胶质瘤细胞；逆转录-聚合酶链式反应（RT-PCR）法和实时荧光定量PCR法测定分化前、后miR-92b基因前体的转录水平。结果 RT-PCR产物的电泳结果显示，在干细胞及分化后的胶质瘤细胞中均出现miR-92b基因前体的目标条带。运用实时荧光定量 PCR法对目的基因进行表达相对定量的2-ΔΔt法，以干细胞miR-92b前体基因为参照，分化后的胶质瘤细胞miR-92b基因前体表达量为2．95，约为干细胞表达量的3倍。结论 miR-92b基因可能在神经胶质瘤干细胞的分化过程中发挥了重要作用。
목적관찰SHG-44신경효질류간세포분화위효질류세포전、후miR-92b기인표체적변화。방법종인효질류SHG-44세포중사선출종류간세포병진행형광면역감정；장종류간세포유도분화위효질류세포；역전록-취합매련식반응（RT-PCR）법화실시형광정량PCR법측정분화전、후miR-92b기인전체적전록수평。결과 RT-PCR산물적전영결과현시，재간세포급분화후적효질류세포중균출현miR-92b기인전체적목표조대。운용실시형광정량 PCR법대목적기인진행표체상대정량적2-ΔΔt법，이간세포miR-92b전체기인위삼조，분화후적효질류세포miR-92b기인전체표체량위2．95，약위간세포표체량적3배。결론 miR-92b기인가능재신경효질류간세포적분화과정중발휘료중요작용。
Objective To compare the expression level of miR-92b in SHanG-44 glioma cells and glioma stem cells .Methods The tumor stem cells were screened from the SHG-44 glioma cells and identified by immunofluorescence .Then it was induced to differentiate .The transcription level of miR-92b was determined by RT-PCR and real-time quantitative PCR before and after differ-entiation .Results The miR-92b expression level in differentiated tumor cells was about 3 times higher than in tumor stem cells . Conclusion T he miR-92b gene is involved in the process of differentiation of glioma stem cells .