CAJ | 학술논문

目的探讨重组血小板衍生生长因子CC（PDGF-CC）对静脉血栓溶解、机化和再通过程的影响。方法建立大鼠下腔静脉血栓模型，将40只雄性SD大鼠随机分为A、B、C、D 4组，建模后24 h A组大鼠注射生理盐水100μL ，B、C、D组大鼠直接静脉腔内注射不同剂量重组PDGF-CC （B组：100 ng/100μL ，C组：200 ng/100μL ，D组：500 ng/100μL ），每组注射量均为100μL。7 d后获取下腔静脉血栓标本，Western blot检测血管内皮生长因子（VEGF）蛋白表达变化，vWF免疫组织化学染色分辨血栓切片中阳性细胞的类型。图像分析系统采集并分析切片 HE染色后静脉管腔面积及血栓面积，计算溶解率。Masson染色评价血栓中胶原纤维和细胞外基质面积，以及新生毛细血管数量，评价血栓机化、再通情况。结果成功建立大鼠下腔静脉血栓模型， PDGF-CC注射后第7天Western blot测定结果表明各组VEGF蛋白均有表达，但C、D组较B组表达明显上调，B组较A组表达明显上调。vWF免疫组织化学染色结果显示阳性细胞类型为血管内皮细胞，证实存在新生血管形成。切片 HE染色及Masson染色结果显示C、D组较其他组血栓溶解更加广泛，血栓机化率较其他组显著提高，同时B组血栓溶解再通机化率高于A组。结论重组PDGF-CC可以通过促血管新生途径显著加快静脉血栓溶解、机化和再通过程。
목적탐토중조혈소판연생생장인자CC（PDGF-CC）대정맥혈전용해、궤화화재통과정적영향。방법건립대서하강정맥혈전모형，장40지웅성SD대서수궤분위A、B、C、D 4조，건모후24 h A조대서주사생리염수100μL ，B、C、D조대서직접정맥강내주사불동제량중조PDGF-CC （B조：100 ng/100μL ，C조：200 ng/100μL ，D조：500 ng/100μL ），매조주사량균위100μL。7 d후획취하강정맥혈전표본，Western blot검측혈관내피생장인자（VEGF）단백표체변화，vWF면역조직화학염색분변혈전절편중양성세포적류형。도상분석계통채집병분석절편 HE염색후정맥관강면적급혈전면적，계산용해솔。Masson염색평개혈전중효원섬유화세포외기질면적，이급신생모세혈관수량，평개혈전궤화、재통정황。결과성공건립대서하강정맥혈전모형， PDGF-CC주사후제7천Western blot측정결과표명각조VEGF단백균유표체，단C、D조교B조표체명현상조，B조교A조표체명현상조。vWF면역조직화학염색결과현시양성세포류형위혈관내피세포，증실존재신생혈관형성。절편 HE염색급Masson염색결과현시C、D조교기타조혈전용해경가엄범，혈전궤화솔교기타조현저제고，동시B조혈전용해재통궤화솔고우A조。결론중조PDGF-CC가이통과촉혈관신생도경현저가쾌정맥혈전용해、궤화화재통과정。
Objective This study aimed to investigate whether platelet-derived growth factor CC could enhance resolution ,or-ganization and recanalization of venous thrombus .Methods The experimental models of deep vein thrombosis in rats were induced as previously described and modified .The rats were randomly divided into four groups according to the dose of PDGF-CC(Group A :0 ng/100 μL ;Group B :100 ng/100 μL ;Group C :200 ng/100 μL and Group D :500 ng/100 μL) .The samples were obtained at 7 days after operation and the expressions of protein VEGF were detected by Western blot analysis ,respectively .Immunohistochemi-cal staining was used to observe the types of the positive cells expressing vWF in thrombus sections .HE staining and Masson stai-ning were used to analyze the area of resolution and organization in venous thrombi .The capillary number was analyzed quantitative-ly by high-power microscope .The area of thrombus and collagen fiber in each section were measured and analyzed with image analy-sis software .Results Animal models of venous thrombogenesis were performed successfully .The data of Western blot analysis demonstrated that the expressions of VEGF protein were significantly increased in group C and D than group B and A ,which were no statistical significance between group C and group D at day 7 after operation .The positive cells of expressing vWF mostly located in the thrombus border and venous wall and the types of the positive cells by immunohistochemical staining were ECs .The resolu-tion rates ,organization rates and recanalization capillary numbers were significantly higher in group C and D than those in group B and A ,and those in group B were higher than those in group A .Conclusion PDGF-CC could enhance resolution ,organization and recanalization of vein thrombi effectively through therapeutic angiogenesis pathway ,which provides a novel strategy for gene thera-py of venous thromboembolism disease .