现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
12期
1282-1284
,共3页
β-细辛醚%PC12细胞%Aβ25-35
β-細辛醚%PC12細胞%Aβ25-35
β-세신미%PC12세포%Aβ25-35
β-asarone%PC12 cell%β-amyloid protein
目的：探讨β-细辛醚对Aβ25-35所致PC12细胞凋亡的抑制作用。方法以Aβ25-35建造体外PC12细胞损伤模型，观察β-细辛醚对其细胞存活率、细胞形态、乳酸脱氢酶（ LDH）的含量和Caspase-3、Caspase-6、Caspase-9 mRNA表达的影响。结果10μmol／L的Aβ25-35与PC12细胞共培养24 h出现明显损伤，细胞活力下降，培养液中LDH含量增多，Caspase-3、Caspase-6、Caspase-9 mRNA表达水平增高。15 mg／L、30 mg／L、60 mg／Lβ-细辛醚可有效改善Aβ25-35引起的PC12细胞形态改变，提高细胞存活力，减少LDH渗漏，明显降低Aβ25-35引起的PC12细胞Caspase-3、Caspase-6、Caspase-9 mRNA表达水平。结论β-细辛醚对Aβ25-35所致PC12细胞损伤有保护作用。
目的：探討β-細辛醚對Aβ25-35所緻PC12細胞凋亡的抑製作用。方法以Aβ25-35建造體外PC12細胞損傷模型，觀察β-細辛醚對其細胞存活率、細胞形態、乳痠脫氫酶（ LDH）的含量和Caspase-3、Caspase-6、Caspase-9 mRNA錶達的影響。結果10μmol／L的Aβ25-35與PC12細胞共培養24 h齣現明顯損傷，細胞活力下降，培養液中LDH含量增多，Caspase-3、Caspase-6、Caspase-9 mRNA錶達水平增高。15 mg／L、30 mg／L、60 mg／Lβ-細辛醚可有效改善Aβ25-35引起的PC12細胞形態改變，提高細胞存活力，減少LDH滲漏，明顯降低Aβ25-35引起的PC12細胞Caspase-3、Caspase-6、Caspase-9 mRNA錶達水平。結論β-細辛醚對Aβ25-35所緻PC12細胞損傷有保護作用。
목적：탐토β-세신미대Aβ25-35소치PC12세포조망적억제작용。방법이Aβ25-35건조체외PC12세포손상모형，관찰β-세신미대기세포존활솔、세포형태、유산탈경매（ LDH）적함량화Caspase-3、Caspase-6、Caspase-9 mRNA표체적영향。결과10μmol／L적Aβ25-35여PC12세포공배양24 h출현명현손상，세포활력하강，배양액중LDH함량증다，Caspase-3、Caspase-6、Caspase-9 mRNA표체수평증고。15 mg／L、30 mg／L、60 mg／Lβ-세신미가유효개선Aβ25-35인기적PC12세포형태개변，제고세포존활력，감소LDH삼루，명현강저Aβ25-35인기적PC12세포Caspase-3、Caspase-6、Caspase-9 mRNA표체수평。결론β-세신미대Aβ25-35소치PC12세포손상유보호작용。
Objective It is to approach the protective effect of β-asarone on PC12 cells apoptosis induced by β-amyloid protein (Aβ25-35).Methods The model of apoptosis of PC12 cells were induced by Aβ25-35 in vitro, the rate of cell viability, the morphological change of PC 12, the consistence of lactate dehydrogenase (LDH), and the expression of Caspase-3, -6, -9 mRNA were observed for assessing the effect on β-asarone in PC12 cells induced by Aβ25-35 .Results Cell survival decrea-sing, LDH leakage and Caspase -3, -6, -9 mRNA expression increasing were happened in PC 12 cells cultured with 10 umol/L pre aggregated Aβ25-35 for 24 hour.15mg/L, 30 mg/L, 60 mg/L β-asarone could increase cell survival , and de-crease the level of lactate dehydrogenase and the expression of Caspase -3, -6, -9 mRNA.Conclusion β-asarone can significantly prevent PC 12 cells apoptosis induced by Aβ25-35 .
