湖南师范大学自然科学学报
湖南師範大學自然科學學報
호남사범대학자연과학학보
ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NORMALIS HUNANENSIS
2013年
4期
74-78
,共5页
曾丹%钟蕾%罗方兴%肖宗琳%谢伟伟%肖克宇
曾丹%鐘蕾%囉方興%肖宗琳%謝偉偉%肖剋宇
증단%종뢰%라방흥%초종림%사위위%초극우
中华鳖%疖疮病%腐皮病%生化鉴定%药敏试验
中華鱉%癤瘡病%腐皮病%生化鑒定%藥敏試驗
중화별%절창병%부피병%생화감정%약민시험
Trionyx sinensis%furunculosis disease%“skin-rotting” disease%biochemical identification%drug sensitivity test
为了鉴定中华鳖“腐皮、疖疮”并发症的病原菌种类,对已呈明显发病症状且频死的20个、体重在100~1000 g之间的中华鳖病样进行了病原菌的分离鉴定,经细菌组织涂片、分离培养与纯化、生理生化特性鉴定和人工感染试验证实,主要致病菌为嗜水气单胞菌( Aeromonas hydrophila)和洛菲不动杆菌( Acinetobacter lwoffi)。药敏试验结果表明,两种病原菌对很多抗生素均存在不同程度的耐药性,而对美洛培南( Meropenem)、复方新诺明( Trimethoprim/Sulfa)等高度敏感,可作为防治该病的首选药物。
為瞭鑒定中華鱉“腐皮、癤瘡”併髮癥的病原菌種類,對已呈明顯髮病癥狀且頻死的20箇、體重在100~1000 g之間的中華鱉病樣進行瞭病原菌的分離鑒定,經細菌組織塗片、分離培養與純化、生理生化特性鑒定和人工感染試驗證實,主要緻病菌為嗜水氣單胞菌( Aeromonas hydrophila)和洛菲不動桿菌( Acinetobacter lwoffi)。藥敏試驗結果錶明,兩種病原菌對很多抗生素均存在不同程度的耐藥性,而對美洛培南( Meropenem)、複方新諾明( Trimethoprim/Sulfa)等高度敏感,可作為防治該病的首選藥物。
위료감정중화별“부피、절창”병발증적병원균충류,대이정명현발병증상차빈사적20개、체중재100~1000 g지간적중화별병양진행료병원균적분리감정,경세균조직도편、분리배양여순화、생리생화특성감정화인공감염시험증실,주요치병균위기수기단포균( Aeromonas hydrophila)화락비불동간균( Acinetobacter lwoffi)。약민시험결과표명,량충병원균대흔다항생소균존재불동정도적내약성,이대미락배남( Meropenem)、복방신낙명( Trimethoprim/Sulfa)등고도민감,가작위방치해병적수선약물。
In order to identify the pathogenic species on complications of “skin-rotting” disease and furuncu-losis disease , an isolation and identification test of pathogenic bacteria experiment from twenty symptoms and dying soft-shelled with weight between 100~1 000 g were conducted .And the main pathogens were verified to be Aero-monas hydrophila and Acinetobacter lwoffi by bacterial organizations smear , cultivation and purification , physiologi-cal and biochemical characterization and artificial infection .The drug-sensitivity tests showed that the two pathogens existed different degrees of resistance to many antibiotics , except Meropenem and Trimethoprim/Sulfa, which were highly sensitive to the two pathogens and could be used as the preferred drug to cure the complications .