CAJ | 학술논문

目的：利用计算机图像处理技术辅助建立一种新的免疫酶组织化学多重染色技术。方法：采用改良的SP法，在同一张切片上进行肝癌组织中3种已知阳性表达的蛋白(乙肝表面抗原HBs、人去唾液酸糖蛋白受体ASGPR和增殖细胞核抗原PCNA)的免疫酶组织化学多重染色，并借助计算机图像处理技术将3张图合成1张能显示多种蛋白表达情况的图。结果：3种蛋白在利用了新的免疫组化多重染色技术进行染色后，阳性信号强烈，效果明显，且不发生抗体的交叉反应，利用计算机图像处理技术处理后的图片也能客观的展示出原来图片中抗原的阳性信号的位置和表达情况，并在同一张图片上展示出3种不同蛋白的表达情况。结论：利用计算机图像处理技术及新的免疫组化多重染色技术能实现在同一张切片上标记多种蛋白的目的。
목적：이용계산궤도상처리기술보조건립일충신적면역매조직화학다중염색기술。방법：채용개량적SP법，재동일장절편상진행간암조직중3충이지양성표체적단백(을간표면항원HBs、인거타액산당단백수체ASGPR화증식세포핵항원PCNA)적면역매조직화학다중염색，병차조계산궤도상처리기술장3장도합성1장능현시다충단백표체정황적도。결과：3충단백재이용료신적면역조화다중염색기술진행염색후，양성신호강렬，효과명현，차불발생항체적교차반응，이용계산궤도상처리기술처리후적도편야능객관적전시출원래도편중항원적양성신호적위치화표체정황，병재동일장도편상전시출3충불동단백적표체정황。결론：이용계산궤도상처리기술급신적면역조화다중염색기술능실현재동일장절편상표기다충단백적목적。
OBJECTIVE:To establish a new technique of multiple immunoenzyme histochemistry staining by using computer image processing technology. METHODS:We stained Hepatitis B virus surface protein (HBs), asiologlycoprotein receptor (ASGPR) and proliferating cell nuclear antigen (PCNA) on the same hepatocellular carcinoma slice using the improved streptavidin-peroxidase method and combined these three figures into one that displayed the expression of these proteins together with the assistance of computer image processing technology. RESULTS:The positive signals of three proteins in the hepatocellular carcinoma slice were clear and strong without antibody cross reactivity. The precessed image could objectively display the exact site of positive signals in the original pictures and show the expression level of these different proteins together in one picture. CONCLUSION:This established method could mark multiple proteins in one slice together.