CAJ | 학술논문

目的：探讨生育力与早期胚胎发育毒性试验中，S D雄鼠交配前的环磷酰胺给药方法，以建立阳性对照模型。方法：SD大鼠按体质量随机分为3组，分别为溶剂对照组、环磷酰胺I组(100 mg/kg)、环磷酰胺II组(20 mg/kg)；环磷酰胺I组和II组分别腹腔注射给予环磷酰胺1次和5次，溶剂对照组给予等量的生理盐水；雄鼠于首次给药后63 d开始交配，交配成功后当天检测阴茎勃起功能，随后处死，进行精子活动度和数量检测，腹主动脉采血后检测双氢睾酮、雌二醇和睾酮激素水平，称量睾丸、附睾、包皮腺、前列腺、精囊腺、提肛肌等的质量并计算脏器系数。结果：2个环磷酰胺组雄鼠主要表现为可逆性脱毛。与溶剂对照组比较，体质量和摄食量均明显降低(P<0.05或0.01)；雄鼠附属生殖器官质量降低(P<0.01)，阴茎勃起潜伏期明显延长(<0.01)，附睾尾精子计数、Ⅰ和Ⅱ级活动精子数及精子活率降低，精子畸形率明显增高(P<0.05或0.01)，但激素水平未见明显异常。结论：给予环磷酰胺对于雄性大鼠的生育力产生明显影响，两种给药方式均可建立生育力与早期胚胎发育毒性试验阳性模型。P
목적：탐토생육력여조기배태발육독성시험중，S D웅서교배전적배린선알급약방법，이건립양성대조모형。방법：SD대서안체질량수궤분위3조，분별위용제대조조、배린선알I조(100 mg/kg)、배린선알II조(20 mg/kg)；배린선알I조화II조분별복강주사급여배린선알1차화5차，용제대조조급여등량적생리염수；웅서우수차급약후63 d개시교배，교배성공후당천검측음경발기공능，수후처사，진행정자활동도화수량검측，복주동맥채혈후검측쌍경고동、자이순화고동격소수평，칭량고환、부고、포피선、전렬선、정낭선、제항기등적질량병계산장기계수。결과：2개배린선알조웅서주요표현위가역성탈모。여용제대조조비교，체질량화섭식량균명현강저(P<0.05혹0.01)；웅서부속생식기관질량강저(P<0.01)，음경발기잠복기명현연장(<0.01)，부고미정자계수、Ⅰ화Ⅱ급활동정자수급정자활솔강저，정자기형솔명현증고(P<0.05혹0.01)，단격소수평미견명현이상。결론：급여배린선알대우웅성대서적생육력산생명현영향，량충급약방식균가건립생육력여조기배태발육독성시험양성모형。P
OBJECTIVE: To establish male rat fertility and early embryo developmental toxicity test (I section) positive control model,cyclophosphamide was given by i.p. before mating. METHODS:Male Sprague-Dawley rats were divided into control group,cyclophosphamide I and cyclophosphamide II groups. Cyclophosphamide groups were given intraperitoneally on day 1 of 9 weeks (100 mg/kg) and the first 5 days of 9 weeks (20 mg/kg). Male rats were sacrificed after mating,the parameters were observed and tested.RESULTS:Positive groups male animals showed reversible depilatory. Animal body weight and then food consumption were decreased significantly when compared with the control group,although no significant differences in the hormone level were found.Weights of auxiliary reproductive organ were significantly decreased,time for penis erection was significantly longer,sperm count and sperm activity ratio were decreased,and sperm malformation rate was increased significantly. CONCLUSION:Cyclophosphamide exeited significant effects on male fertility,and both treatment methods could be used to establish positive fertility and early embryo developmental toxicity test model.