CAJ | 학술논문

目的：观察大鼠连续经皮给予大剂量乳酸钠后的毒性靶器官及其损害的可逆性。方法：设溶剂对照组和乳酸钠高、中、低3个剂量组(分别为2000、1000和500 mg/kg)，实验组大鼠连续经皮涂抹乳酸钠90 d，溶剂对照组给予蒸馏水。于第7周、第13周和停止染毒后4周，每组剖杀部分大鼠做血液学、生化学、眼科、尿液检测，测量脏器湿重和脏器系数，并进行解剖病理学观察。结果：染毒第13周各剂量组大鼠血液钠离子、钙离子浓度升高，钾离子、氯离子浓度降低，膀胱腔内存在蛋白样物质聚集；高剂量组大鼠尿液中蛋白和胆红素浓度升高，比重降低。停止染毒后4周高剂量组大鼠血液钠离子浓度仍然较高。结论：乳酸钠染毒后大鼠出现电解质轻度紊乱和尿液异常、尿蛋白增加等变化，因此乳酸钠对大鼠具有一定的毒性作用。
목적：관찰대서련속경피급여대제량유산납후적독성파기관급기손해적가역성。방법：설용제대조조화유산납고、중、저3개제량조(분별위2000、1000화500 mg/kg)，실험조대서련속경피도말유산납90 d，용제대조조급여증류수。우제7주、제13주화정지염독후4주，매조부살부분대서주혈액학、생화학、안과、뇨액검측，측량장기습중화장기계수，병진행해부병이학관찰。결과：염독제13주각제량조대서혈액납리자、개리자농도승고，갑리자、록리자농도강저，방광강내존재단백양물질취집；고제량조대서뇨액중단백화담홍소농도승고，비중강저。정지염독후4주고제량조대서혈액납리자농도잉연교고。결론：유산납염독후대서출현전해질경도문란화뇨액이상、뇨단백증가등변화，인차유산납대대서구유일정적독성작용。
OBJECTIVE:To study the subchronic dermal toxicity of sodium lactate in rats,and analyze the target organ damage and its reversibility. METHODS:Sodium lactate was given to rats by transdermal administration at th thdose of 2 000,1 000 and 500 mg/kg for 90 days. Some rats from each group were sacrificed at 7 ,13 week and 4 weeks after recovery,blood count and biochemistry were tested,ophthalmic and urinalysis tests were performed,wet weight of individual organs and the coefficient values were determined,and anatomic pathology were observed. RESULTS:With+ - + 2+all treatment doses,K ,Cl decreased and Na ,Ca increased,protein precipitation was found in the bladder lumen. High dose group showed urinary protein and bilirubin concentration increased,specific gravity reduced. The above had+dose-response relationship. Na values in high dose group remained elevated in the recovery stage. CONCLUSION:Sodium lactate showed significant toxic effects in rats as determined by mild disturbance of electrolyte and abnormal urinary protein.