福建体育科技
福建體育科技
복건체육과기
FUJIAN SPORTS SCIENCE AND TECHNOLOGY
2013年
4期
31-33
,共3页
罗凯%王植元%张迪迪%刘晨涛
囉凱%王植元%張迪迪%劉晨濤
라개%왕식원%장적적%류신도
抗阻力运动%PI3K%Akt
抗阻力運動%PI3K%Akt
항조력운동%PI3K%Akt
Resistance Training%PI3K%Akt
探讨中等强度抗阻力训练对骨骼肌PI3K基因和Akt基因表达的影响。方法：18只健康的SD大鼠随机分为安静组、有氧运动组和抗阻力训练组，每组6只。训练组采用Bedford等人跑台训练方案。8周训练结束后将大鼠安静状态下处死，取股四头肌，液氮冻存。采用Reverse Transcription -PCR方法测定大鼠股四头肌PI3K mRNA和AKt mRNA表达。结果：8周训练后，抗阻力运动组大鼠股四头肌PI3K mR-NA的表达较安静对照组和有氧运动组显著增多（ p＜0．05）。 Akt mRNA表达较有氧运动组无显著差异（p＞0．05），较安静对照组有显著增长（p＜0．05）。结论：抗阻力跑台运动和有氧运动均可促进大鼠股四头肌PI3K mRNA和Akt mRNA表达，推测抗阻力运动可增强PI3K/Akt信号途径中信号转导，对促进糖原和蛋白合成有促进作用。
探討中等彊度抗阻力訓練對骨骼肌PI3K基因和Akt基因錶達的影響。方法：18隻健康的SD大鼠隨機分為安靜組、有氧運動組和抗阻力訓練組，每組6隻。訓練組採用Bedford等人跑檯訓練方案。8週訓練結束後將大鼠安靜狀態下處死，取股四頭肌，液氮凍存。採用Reverse Transcription -PCR方法測定大鼠股四頭肌PI3K mRNA和AKt mRNA錶達。結果：8週訓練後，抗阻力運動組大鼠股四頭肌PI3K mR-NA的錶達較安靜對照組和有氧運動組顯著增多（ p＜0．05）。 Akt mRNA錶達較有氧運動組無顯著差異（p＞0．05），較安靜對照組有顯著增長（p＜0．05）。結論：抗阻力跑檯運動和有氧運動均可促進大鼠股四頭肌PI3K mRNA和Akt mRNA錶達，推測抗阻力運動可增彊PI3K/Akt信號途徑中信號轉導，對促進糖原和蛋白閤成有促進作用。
탐토중등강도항조력훈련대골격기PI3K기인화Akt기인표체적영향。방법：18지건강적SD대서수궤분위안정조、유양운동조화항조력훈련조，매조6지。훈련조채용Bedford등인포태훈련방안。8주훈련결속후장대서안정상태하처사，취고사두기，액담동존。채용Reverse Transcription -PCR방법측정대서고사두기PI3K mRNA화AKt mRNA표체。결과：8주훈련후，항조력운동조대서고사두기PI3K mR-NA적표체교안정대조조화유양운동조현저증다（ p＜0．05）。 Akt mRNA표체교유양운동조무현저차이（p＞0．05），교안정대조조유현저증장（p＜0．05）。결론：항조력포태운동화유양운동균가촉진대서고사두기PI3K mRNA화Akt mRNA표체，추측항조력운동가증강PI3K/Akt신호도경중신호전도，대촉진당원화단백합성유촉진작용。
To explore the influence of the moderate intensity resistance training on the expression of PI 3K and Akt gene in skeletal musle .Methods: 18 healthy Sprague -Dawlev rats were randomly distributed into three groups:normal control group ( NC, n =6 ); aerobic exercise group ( AE, n =6 ); medium resistance training group (MRT, n=6).Quadriceps femoris muscles were excised after 8 weeks training and freezed in liquid nitrogen and analyzed by reverse transcription PCR .Results:After 8 weeks resistance training , the expression of PI3K mRNA in medium resistance training rats was significantly different from that in normal control group and aerobic exercise group rats (p<0.05).And the expression of Akt mRNA has no significant difference between resistance training group and aerobic exercise group rats (p>0.05), but medium resistance training group was markedly higher than normal control group in the expression of Akt mRNA (p<0.05).Conclusion:The expression levels of PI3K mRNA and Akt mRNA in quadriceps femoris muscles can be improved by resistance training and aerobic exercise . It conjectures that treadmill resistance exercise can strengthen the signal transduction of PI 3K/Akt signal pathway , resulting in promote glycogen and protein synthesis .
