CAJ | 학술논문

目的：探讨表没食子儿茶素没食子酸酯[(-)epigallocatechin gallate ，EGCG]对人成淋巴细胞株错配修复基因hMLH1和hMSH2 mRNA表达水平的影响。方法：将正常人成淋巴细胞株GM12593和乳腺癌患者成淋巴细胞株GM13705分别置于含有0、5、10、20μmol/L EGCG的RPMI-1640中进行6 d干预培养后，采用实时荧光定量PCR (FQ-PCR)技术检测干预前后错配修复基因hMLH1和hMSH2 mRNA表达水平的变化。结果：经20μmol/L EGCG干预培养6 d后，GM12593细胞hMLH1与hMSH2 mRNA表达水平均显著高于其他浓度组(P均<0.05)，且显著高于同等浓度时GM13705细胞中上述2个基因的表达水平(P<0.05)；EGCG对GM13705目标基因的表达无显著影响(P>0.05)。结论：EGCG具有上调正常人成淋巴细胞株hMLH1与hMSH2 mRNA表达水平的潜力，可能通过增加错配修复起始复合物的数量，来帮助错配修复机制的启动，维护基因组的稳定性。
목적：탐토표몰식자인다소몰식자산지[(-)epigallocatechin gallate ，EGCG]대인성림파세포주착배수복기인hMLH1화hMSH2 mRNA표체수평적영향。방법：장정상인성림파세포주GM12593화유선암환자성림파세포주GM13705분별치우함유0、5、10、20μmol/L EGCG적RPMI-1640중진행6 d간예배양후，채용실시형광정량PCR (FQ-PCR)기술검측간예전후착배수복기인hMLH1화hMSH2 mRNA표체수평적변화。결과：경20μmol/L EGCG간예배양6 d후，GM12593세포hMLH1여hMSH2 mRNA표체수평균현저고우기타농도조(P균<0.05)，차현저고우동등농도시GM13705세포중상술2개기인적표체수평(P<0.05)；EGCG대GM13705목표기인적표체무현저영향(P>0.05)。결론：EGCG구유상조정상인성림파세포주hMLH1여hMSH2 mRNA표체수평적잠력，가능통과증가착배수복기시복합물적수량，래방조착배수복궤제적계동，유호기인조적은정성。
OBJECTIVE: To investigate the effect of (-) epigallocatechin gallate(EGCG) on mRNA expressions of hMLH1 and hMSH2 in human lymphoblast cell lines. METHODS:Total RNA was isolated from GM12593 and GM13705 cells treated with EGCG(0,5,10,20 μmol/L) for 6 days. Real-time fluorecence quantification PCR(FQ-PCR) was conducted to measure the mRNA expression levels of hMLH1 and hMSH2. RESULTS:The mRNA expressions of hMLH1 and hMSH2 significantly increased at 20μmol/L EGCG in GM12593 cells,and were significantly higher than equal concentrations in GM13705 cells. The effect of EGCG on expressions of target genes were insignificant in GM13705 cells. CONCLUSION: EGCG had the potential to up-regulate the expressions of hMLH1 and hMSH2, contributed to the promotion of mismatch repair and maintaining genomic stability in normal human lymphoblast cell line GM12593.