CAJ | 학술논문

目的：检测染色体8q21~24区域E2F5和MYC癌基因在前列腺癌(prostate cancer，PCa)细胞系、癌组织及癌旁组织中的表达，分析其表达水平与临床病理参数的关系，并探讨其意义。方法：采用DNA qPCR检测PCa细胞系Du145、LNCap、PC3和PCa组织标本中E2F5和MYC基因DNA拷贝数，Western blot和免疫组织化学技术检测前列腺癌和癌旁组织中E2F5和MYC蛋白的表达，结合患者临床病理参数进一步验证和分析蛋白表达及其临床意义。结果：DNA qPCR结果显示，PCa组织中E2F5和MYC DNA拷贝数较癌旁组织明显增加(P<0.05或P<0.01)，但在癌细胞系中其表达与对照组比较无明显变化(P>0.05)。Western blot显示，在PCa组织中E2F5和MYC蛋白表达水平显著高于癌旁组织(P<0.05或P<0.01)。免疫组化染色发现，与癌旁良性组织相比，E2F5和MYC蛋白表达显著增加(P均<0.01)。而且， E2F5的过表达与高的Gleason评分(P<0.01)、临床分期(P=0.01)、阳性转移(P<0.01)、生化复发阳性(P<0.01)相关。MYC的过表达与阳性转移(P=0.02)、生化复发阳性(P=0.02)相关。且PCa组织中E2F5和MYC蛋白表达两者呈正相关关系(rs=0.5，P<0.01)。结论：E2F5和MYC的表达增加可能与PCa的发生发展密切相关，E2F5可能是PCa新的潜在候选分子标志物。
목적：검측염색체8q21~24구역E2F5화MYC암기인재전렬선암(prostate cancer，PCa)세포계、암조직급암방조직중적표체，분석기표체수평여림상병리삼수적관계，병탐토기의의。방법：채용DNA qPCR검측PCa세포계Du145、LNCap、PC3화PCa조직표본중E2F5화MYC기인DNA고패수，Western blot화면역조직화학기술검측전렬선암화암방조직중E2F5화MYC단백적표체，결합환자림상병리삼수진일보험증화분석단백표체급기림상의의。결과：DNA qPCR결과현시，PCa조직중E2F5화MYC DNA고패수교암방조직명현증가(P<0.05혹P<0.01)，단재암세포계중기표체여대조조비교무명현변화(P>0.05)。Western blot현시，재PCa조직중E2F5화MYC단백표체수평현저고우암방조직(P<0.05혹P<0.01)。면역조화염색발현，여암방량성조직상비，E2F5화MYC단백표체현저증가(P균<0.01)。이차， E2F5적과표체여고적Gleason평분(P<0.01)、림상분기(P=0.01)、양성전이(P<0.01)、생화복발양성(P<0.01)상관。MYC적과표체여양성전이(P=0.02)、생화복발양성(P=0.02)상관。차PCa조직중E2F5화MYC단백표체량자정정상관관계(rs=0.5，P<0.01)。결론：E2F5화MYC적표체증가가능여PCa적발생발전밀절상관，E2F5가능시PCa신적잠재후선분자표지물。
OBJECTIVE:The aim of this study was to identify the expressions of E2F5 and MYC gene located in chromosomal region 8q21–24 in prostate cancer (PCa),adjacent benign prostate tissues and PCa cell lines,to analyze their expression levels and to explore theirs significances in PCa. METHODS:DNA qPCR analysis was carried out to evaluate the copy number changes of E2F5 and MYC genes in PCa tissues and PCa cell lines. Western blot and immunohistochemical staining were used to assess expressions of E2F5 and MYC in PCa and BPH tissues,and analyzed together with clinical pathological parameters. RESULTS:DNA qPCR revealed a significant copy number gain of E2F5 and MYC in PCa tissues but not in PCa cell lines (P<0.05 or P<0.01). In addition,Western blot analysis and immunohistochemical staining both found the significantly higher expression of E2F5 and MYC proteins in PCa tissues than those in adjacent benign specimens (all P<0.01). Moreover,the overexpression of E2F5 protein was significantly associated with a high Gleason score (P<0.01),an advanced clinical stage (P=0.01),a positive metastasis (P<0.01) and biochemical recurrence (P<0.01). The overexpression of MYC was more frequently found in PCa tissues with positive metastasis (P=0.02) and biochemical recurrence (P=0.02). Interestingly,there was a close correlation in the expression level of MYC in PCa tissues with that of E2F5 (rs=0.5,P<0.01). CONCLUSION:Overexpressions of E2F5 and MYC may play an important role in the development and progression of PCa. E2F5 may be a novel potential candidate marker for malignant PCa.