海洋科学
海洋科學
해양과학
MARINE SCIENCES
2013年
7期
63-71
,共9页
凡纳滨对虾(Litopenaeus vannamei)%Ca2+%Mg2+%盐度%消化酶%ATP酶%免疫类酶
凡納濱對蝦(Litopenaeus vannamei)%Ca2+%Mg2+%鹽度%消化酶%ATP酶%免疫類酶
범납빈대하(Litopenaeus vannamei)%Ca2+%Mg2+%염도%소화매%ATP매%면역류매
Litopenaeus vannamei%Ca2+%Mg2+%salinity%digestive enzymes%ATP enzymes%immune enzymes
为探讨 Ca2+、Mg2+、盐度对凡纳滨对虾体内代谢酶的相对独立作用和相互影响,进而为提高凡纳滨对虾生长力和免疫力提供理论依据,本实验采取L49(78)安排7水平Ca2+、Mg2+、盐度, L8(27)安排2水平Ca2+、Mg2+、盐度,开展60 d凡纳滨对虾(Litopenaeus vannamei)养殖试验,通过比较对虾体内消化酶、ATP酶及免疫类酶的活性以分析Ca2+、Mg2+、盐度对凡纳滨对虾生长力和免疫力的影响。结果表明：水体中Ca2+、Mg2+、盐度对消化酶具有显著影响(P<0.05),其中与对虾消化吸收联系最紧密的蛋白酶中,盐度对胃蛋白酶影响显著,盐度为10时酶活最高, Ca2+、盐度对类胰蛋白酶影响显著, Ca2+为200 mg/L,盐度为20时,酶活最高； Ca2+、Mg2+、盐度对 ATP 酶具有显著影响(P<0.05),其中对Na+-K+-ATP酶都有显著影响, Ca2+为300 mg/L, Mg2+为500 mg/L,盐度为30时酶活最高, Ca2+、Mg2+对Mg2+-ATP酶具有显著影响, Ca2+为200 mg/L, Mg2+为500 mg/L时酶活最高, Ca2+对Ca2+-ATP酶具有显著影响, Ca2+为200、300 mg/L时酶活最高； Ca2+、Mg2+、盐度对凡纳滨对虾体内免疫酶具有显著影响(P<0.05), Ca2+、Mg2+、盐度对ACP都有显著影响, Ca2+为100 mg/L, Mg2+为150 mg/L,盐度为30时酶活最高, Mg2+对AKP具有显著影响,在150 mg/L时酶活最高, Ca2+、盐度对SOD酶活具有显著影响, Ca2+为100 mg/L,盐度为35时酶活最高；Ca2+、Mg2+、盐度间的交互作用对体内代谢酶也有一定影响。
為探討 Ca2+、Mg2+、鹽度對凡納濱對蝦體內代謝酶的相對獨立作用和相互影響,進而為提高凡納濱對蝦生長力和免疫力提供理論依據,本實驗採取L49(78)安排7水平Ca2+、Mg2+、鹽度, L8(27)安排2水平Ca2+、Mg2+、鹽度,開展60 d凡納濱對蝦(Litopenaeus vannamei)養殖試驗,通過比較對蝦體內消化酶、ATP酶及免疫類酶的活性以分析Ca2+、Mg2+、鹽度對凡納濱對蝦生長力和免疫力的影響。結果錶明：水體中Ca2+、Mg2+、鹽度對消化酶具有顯著影響(P<0.05),其中與對蝦消化吸收聯繫最緊密的蛋白酶中,鹽度對胃蛋白酶影響顯著,鹽度為10時酶活最高, Ca2+、鹽度對類胰蛋白酶影響顯著, Ca2+為200 mg/L,鹽度為20時,酶活最高； Ca2+、Mg2+、鹽度對 ATP 酶具有顯著影響(P<0.05),其中對Na+-K+-ATP酶都有顯著影響, Ca2+為300 mg/L, Mg2+為500 mg/L,鹽度為30時酶活最高, Ca2+、Mg2+對Mg2+-ATP酶具有顯著影響, Ca2+為200 mg/L, Mg2+為500 mg/L時酶活最高, Ca2+對Ca2+-ATP酶具有顯著影響, Ca2+為200、300 mg/L時酶活最高； Ca2+、Mg2+、鹽度對凡納濱對蝦體內免疫酶具有顯著影響(P<0.05), Ca2+、Mg2+、鹽度對ACP都有顯著影響, Ca2+為100 mg/L, Mg2+為150 mg/L,鹽度為30時酶活最高, Mg2+對AKP具有顯著影響,在150 mg/L時酶活最高, Ca2+、鹽度對SOD酶活具有顯著影響, Ca2+為100 mg/L,鹽度為35時酶活最高；Ca2+、Mg2+、鹽度間的交互作用對體內代謝酶也有一定影響。
위탐토 Ca2+、Mg2+、염도대범납빈대하체내대사매적상대독립작용화상호영향,진이위제고범납빈대하생장력화면역력제공이론의거,본실험채취L49(78)안배7수평Ca2+、Mg2+、염도, L8(27)안배2수평Ca2+、Mg2+、염도,개전60 d범납빈대하(Litopenaeus vannamei)양식시험,통과비교대하체내소화매、ATP매급면역류매적활성이분석Ca2+、Mg2+、염도대범납빈대하생장력화면역력적영향。결과표명：수체중Ca2+、Mg2+、염도대소화매구유현저영향(P<0.05),기중여대하소화흡수련계최긴밀적단백매중,염도대위단백매영향현저,염도위10시매활최고, Ca2+、염도대류이단백매영향현저, Ca2+위200 mg/L,염도위20시,매활최고； Ca2+、Mg2+、염도대 ATP 매구유현저영향(P<0.05),기중대Na+-K+-ATP매도유현저영향, Ca2+위300 mg/L, Mg2+위500 mg/L,염도위30시매활최고, Ca2+、Mg2+대Mg2+-ATP매구유현저영향, Ca2+위200 mg/L, Mg2+위500 mg/L시매활최고, Ca2+대Ca2+-ATP매구유현저영향, Ca2+위200、300 mg/L시매활최고； Ca2+、Mg2+、염도대범납빈대하체내면역매구유현저영향(P<0.05), Ca2+、Mg2+、염도대ACP도유현저영향, Ca2+위100 mg/L, Mg2+위150 mg/L,염도위30시매활최고, Mg2+대AKP구유현저영향,재150 mg/L시매활최고, Ca2+、염도대SOD매활구유현저영향, Ca2+위100 mg/L,염도위35시매활최고；Ca2+、Mg2+、염도간적교호작용대체내대사매야유일정영향。
The L49(78) orthogonal experimental design was used to investigate the three factors Ca2+, Mg2+and salinity in water, and seven levels of each factor were based. The L49(78) orthogonal experimental design was used to investigate the three factors Ca2+, Mg2+and salinity in water, and two levels of each factor were based. In order to analyze the effect of Ca2+, Mg2+and salinity in water on the growth performance and immunity of L. vannamei, the digestive enzymes, ATP enzyme and immune enzymes were compared during the 60 d culture cycle. Results showed that Ca2+, and salinity had significant effect on digestive enzymes(P<0.05), the salinity had significant effect on pepsin, and the Ca2+and salinity had significant effect on tgryptase. Ca2+, Mg2+and salinity had significant effect on ATP enzymes(P<0.05), and Ca2+, Mg2+and salinity had significant effect on Na+-K+-ATPase, with maximum activ-ity at Ca2+ 300 mg/L, Mg2+ 500 mg/L and salinity 30. Ca2+ and Mg2+ had significant effect on Mg2+-ATPase and Ca2+ had significant effect on Ca2+-ATPase. Ca2+, Mg2+ and salinity had significant effect on immune en-zymes(P<0.05), and Ca2+, Mg2+and salinity had significant effect on ACP, with maximum activity at Ca2+100 mg/L, Mg2+150 mg/L and salinity 30. Mg2+had significant effect on AKP with maximum activity at 150 mg/L. Ca2+and salinity had significant effect on SOD, with maximum activity at Ca2+100 mg/L, salinity 35. The interaction of Ca2+, Mg2+, and salinity also had some impact on metabolic enzymes of L. vannamei.
