CAJ | 학술논문

栅通过油脂分析、光合放氧、叶绿素荧光等手段,研究了缺氮处理对栅藻(Scenedesmus dimorphus)油脂含量、油脂组分以及光合作用影响。结果显示,总脂含量由22.4%±0.6%提高到36.3%±0.7%,其中甘油三酯含量由4.6%±1.2%提高到68.3%±2.5%而磷脂含量由92.8%±1.6%降低到26.8%±2.0%。缺氮处理1d后,叶绿素含量明显下降,呼吸速率明显升高。缺氮处理初始阶段,栅藻光系统Ⅱ最大光能转化效率(Fv/Fm)和非光化学猝灭系数(NPQ)维持稳定,4 d后Fv/Fm显著降低而NPQ显著升高。上述变化可能是光能过剩引起的防御反应,也可能是自身代谢模式发生转变的结果而与光能耗散无关。
책통과유지분석、광합방양、협록소형광등수단,연구료결담처리대책조(Scenedesmus dimorphus)유지함량、유지조분이급광합작용영향。결과현시,총지함량유22.4%±0.6%제고도36.3%±0.7%,기중감유삼지함량유4.6%±1.2%제고도68.3%±2.5%이린지함량유92.8%±1.6%강저도26.8%±2.0%。결담처리1d후,협록소함량명현하강,호흡속솔명현승고。결담처리초시계단,책조광계통Ⅱ최대광능전화효솔(Fv/Fm)화비광화학졸멸계수(NPQ)유지은정,4 d후Fv/Fm현저강저이NPQ현저승고。상술변화가능시광능과잉인기적방어반응,야가능시자신대사모식발생전변적결과이여광능모산무관。
The microalga Scenedesmus dimorphus could be potentially used as feedstock for biodiesel production. However, the changes in photosynthesis during lipid accumulation are still unclear for this organism. Herein, the effects of nitrogen starvation on the lipid content, lipid component and photosynthesis of Scenedesmus dimorphus were studied using lipid analyzing, photosynthetic oxygen evolution and chlorophyll fluorescence methods,. The results showed that the total lipid content was increased from (22.4 ± 0.6)% to (36.3 ± 0.7)% during the nitrogen starvation, companied by the increase of triacylglycerol from (4.6 ± 1.2)%to (68.3 ± 2.5)%and decrease of phos-pholipid from (92.8 ± 1.6)%to (26.8 ± 2.0)%. The chlorophyll content was decreased steeply while the respiration rate was increased after 1 day nitrogen starvation treatment. The maximum photo-chemical efficiency of photosys-temⅡ(Fv/Fm) and non-photochemical quenching co-efficiency (NPQ) were kept in a stable level during the first several days of nitrogen starvation, and then Fv/Fm decreased while NPQ increased significantly after 4 days treat-ment. All these changes in photosynthesis might be due to 1) the defensive reaction against the excess light energy, or 2) non-related with the energy dissipation but due to the shift from vegetative growth to secondary metabolism.