CAJ | 학술논문

以初始体质量为(2.74±0.17)g的刺参(Apostichopus japonicus)为研究对象，在室内养殖池进行60 d饲喂实验，研究饲料中添加乳酸菌制剂和乳酸菌代谢产物对刺参肠道菌群、部分免疫指标及生长的影响。在基础饲料中分别添加乳酸菌制剂和乳酸菌代谢产物各2 mL/m3(按饲养水体折算)，配制2种实验饲料。实验结果显示：与对照组比，饲料中添加乳酸菌与乳酸菌代谢产物均显著降低了刺参肠道弧菌数及大肠杆菌数(P＜0.05)；饲料中添加乳酸菌及其代谢产物能使刺参肠道酸性磷酸酶和碱性磷酸酶活力显著升高，溶菌酶活性有所提升，其中乳酸菌制剂实验组与代谢产物实验组免疫酶活力差异不显著；两实验组刺参的特定生长率显著高于对照组(P＜0.05)。上述结果表明，乳酸菌制剂与乳酸菌代谢产物对刺参的肠道菌群平衡、免疫力及生长均具有良好的促进作用，且两者之间差异不显著。
이초시체질량위(2.74±0.17)g적자삼(Apostichopus japonicus)위연구대상，재실내양식지진행60 d사위실험，연구사료중첨가유산균제제화유산균대사산물대자삼장도균군、부분면역지표급생장적영향。재기출사료중분별첨가유산균제제화유산균대사산물각2 mL/m3(안사양수체절산)，배제2충실험사료。실험결과현시：여대조조비，사료중첨가유산균여유산균대사산물균현저강저료자삼장도호균수급대장간균수(P＜0.05)；사료중첨가유산균급기대사산물능사자삼장도산성린산매화감성린산매활력현저승고，용균매활성유소제승，기중유산균제제실험조여대사산물실험조면역매활력차이불현저；량실험조자삼적특정생장솔현저고우대조조(P＜0.05)。상술결과표명，유산균제제여유산균대사산물대자삼적장도균군평형、면역력급생장균구유량호적촉진작용，차량자지간차이불현저。
A 60-day feeding experiment was conducted to evaluate the effects of lactic acid bacteria and its me-tabolites on bacteria of intestine, nonspecific immunity and growth of juvenile sea cucumber with initial body weight of (2.74 ± 0.17) g. Lactic acid bacteria and metabolites were added in the basal diet with 2 mL/m3 (translated by farming water). Compared with control group, the lactic acid bacteria and metabolites added to the feed signifi-cantly reduced the number of intestinal Vibrio and E. coli (P<0.05). The diet containing lactic acid bacteria and metabolites can increase the activities of acid phosphatase, alkaline phosphatase, and lysozyme significantly. There was no significant difference in activities of immune enzyme between the experimental groups. The results showed that the specific growth rate (RSG) of sea cucumber in the experimental groups were significantly higher (P<0.05) than that of the control group. These results indicated that both lactic acid bacteria and metabolites play an impor-tant role on intestinal microflora balance, nonspecific immunity and growth of sea cucumber, but the difference between the experimental groups was not significant. The metabolites may play a major role of probiotics in the process of sea cucumber farming.