海洋科学
海洋科學
해양과학
MARINE SCIENCES
2013年
7期
1-6
,共6页
孙璐%邱天龙%许强%刘辉%林承刚%杨红生
孫璐%邱天龍%許彊%劉輝%林承剛%楊紅生
손로%구천룡%허강%류휘%림승강%양홍생
仿刺参(Apostichopus japonicus)%体外标记%标签保留率
倣刺參(Apostichopus japonicus)%體外標記%標籤保留率
방자삼(Apostichopus japonicus)%체외표기%표첨보류솔
Apostichopus japonicus%external tagging%retention rate
为筛选出仿刺参(Apostichopus japonicus)体外标记方法,研究了体腔植入标记物法、体壁半包埋法、棘部及体壁穿刺法、体壁胶粘法和石灰环嵌套法。研究结果表明,前4种方法标记保留时间均未超过2 d,且有严重排异反应,影响活动和摄食,难以满足标记要求,体壁胶粘法保留时间可达2 d,且活动摄食正常,可用于短期标记。使用石灰环嵌套法标记小规格仿刺参10 d后标签保留率达到75.8%,标记大规格仿刺参3个月后标签保留率可达93%,初步确定石灰环嵌套法可作为仿刺参体外长效标记的方法。
為篩選齣倣刺參(Apostichopus japonicus)體外標記方法,研究瞭體腔植入標記物法、體壁半包埋法、棘部及體壁穿刺法、體壁膠粘法和石灰環嵌套法。研究結果錶明,前4種方法標記保留時間均未超過2 d,且有嚴重排異反應,影響活動和攝食,難以滿足標記要求,體壁膠粘法保留時間可達2 d,且活動攝食正常,可用于短期標記。使用石灰環嵌套法標記小規格倣刺參10 d後標籤保留率達到75.8%,標記大規格倣刺參3箇月後標籤保留率可達93%,初步確定石灰環嵌套法可作為倣刺參體外長效標記的方法。
위사선출방자삼(Apostichopus japonicus)체외표기방법,연구료체강식입표기물법、체벽반포매법、극부급체벽천자법、체벽효점법화석회배감투법。연구결과표명,전4충방법표기보류시간균미초과2 d,차유엄중배이반응,영향활동화섭식,난이만족표기요구,체벽효점법보류시간가체2 d,차활동섭식정상,가용우단기표기。사용석회배감투법표기소규격방자삼10 d후표첨보류솔체도75.8%,표기대규격방자삼3개월후표첨보류솔가체93%,초보학정석회배감투법가작위방자삼체외장효표기적방법。
In order to select an external tagging method for sea cucumber Apostichopus japonicus, we have devel-oped and evaluated six methods including body cavity implantation, somatic embedding, spine and somatic piercing, somatic affix and calcareous ring nesting. The results indicated that the first four methods were not suitable for tag-ging, with the tags kept less than 2 days, causing a rejection reaction, affecting the sea cucumber’s moving and feeding. In the somatic affix method, the tags were kept for 2 days without affecting the sea cucumber’s behavior, which made it a suitable short-time tagging method. The retention rates of tags were 75.8%and 93%in 10 days and 3 months for juvenile and grown sea cucumber, respectively, using calcareous ring nesting. So the calcareous ring nesting method could be an acceptable method of external tagging of sea cucumber.