CAJ | 학술논문

以脊尾白虾( Exopalaemon carinicauda)糠虾幼体为实验材料，研究底泥浸出液对抗氧化解毒酶和基因表达的影响。用长江口水域的底泥浸出液与过滤海水按等比例间距稀释成4个体积浓度水平（1∶4，1∶2，1∶1，1.5∶1），以过滤海水为对照组，每一个浓度组设3个平行样本，进行20 d的毒性实验。结果表明：实验进行20 d，底泥浸出液对糠虾幼体肌肉组织CAT酶活性有一定的诱导作用，浸出液浓度与CAT酶活性表现出正相关的剂量效应关系。实验开始10 d，不同浸出液胁迫下，GST酶活性均出现增加受到诱导，实验20 d后，GST酶活性下降受到抑制作用，但不同浸出液浓度胁迫下抑制和诱导效应关系不明显。虾类肌肉组织3种基因（CAT、GSTD和HSP70）表达量基本都表现为实验开始10 d时升高，而实验20 d后表达量下降，仅为10 d数值的百分之一左右，表明底泥浸出液对CAT、GSTD和HSP70的3种基因的mRNA转录水平表达在一定时间内有一定的诱导作用，但胁迫时间过长时，则产生较明显的抑制作用。
이척미백하( Exopalaemon carinicauda)강하유체위실험재료，연구저니침출액대항양화해독매화기인표체적영향。용장강구수역적저니침출액여과려해수안등비례간거희석성4개체적농도수평（1∶4，1∶2，1∶1，1.5∶1），이과려해수위대조조，매일개농도조설3개평행양본，진행20 d적독성실험。결과표명：실험진행20 d，저니침출액대강하유체기육조직CAT매활성유일정적유도작용，침출액농도여CAT매활성표현출정상관적제량효응관계。실험개시10 d，불동침출액협박하，GST매활성균출현증가수도유도，실험20 d후，GST매활성하강수도억제작용，단불동침출액농도협박하억제화유도효응관계불명현。하류기육조직3충기인（CAT、GSTD화HSP70）표체량기본도표현위실험개시10 d시승고，이실험20 d후표체량하강，부위10 d수치적백분지일좌우，표명저니침출액대CAT、GSTD화HSP70적3충기인적mRNA전록수평표체재일정시간내유일정적유도작용，단협박시간과장시，칙산생교명현적억제작용。
The object of this study was to evaluate the effects of lixivium from ooze mud in the Changjiang estuary on antioxidant detoxifying enzymes activity in muscles of Exopalaemon carinicauda Mysis larvae and expressions of relative genes. The mysis larvae were exposed to different the volume ratio of lixivium from ooze mud and natural seawater levels:0 (natural seawater or control), 1∶4, 1∶2, 1∶1 and 1.5∶1 for 20 d and sampled randomly at 0 d,10 d and 20 d. The results indicated that in order to reduce the harm to the organism, antioxidant detoxifying enzymes like CAT and GST were response to overcome harmful environment and preventing toxicosis after forced by the lixivium from oonze mud. When Mysis larvae were exposed the lixivium, the activity of CAT would significantly rise in muscles. The concentration of the lixivium had significant positive correlation with the activity of CAT. Moreover, the activity of GST would significantly rise in 10 d, while the active was inhibited in 20 d. The dose-response relationship was not obvious. Detected the mRNA expression level of CAT, GST and HSP70, we found that the CAT, GST and HSP70 expression level in muscles of Exopalaemon carinicauda mysis larvae was all increasing in 10 d, while the expression level was decreasing in 20d. There was synchronization between the changes of enzyme activities of CAT and mRNA expression level changes of relative genes.