CAJ | 학술논문

为探究灰树花多糖对吉富罗非鱼免疫细胞和抗氧化酶活性的影响，在饲料中添加不同质量分数的灰树花多糖。将规格相同、质量140～150 g的吉富罗非鱼随机分为3个处理组，饲养在装有温度为26～30℃的曝气自来水的水箱（60 cm ×42 cm ×38 cm ）中，投喂添加质量分数为0、0．05％、0．20％灰树花多糖的饲料，饲喂一个月。试验结果表明，灰树花多糖能增加吉富罗非鱼红细胞和白细胞数量，但对白细胞吞噬活性无明显影响。灰树花多糖对血清、肝脏中过氧化物酶活性有显著促进作用，且表现出明显的剂量效应；对超氧化物歧化酶活性表现出一定的低促高抑现象；过氧化氢酶活性受多糖的促进作用随时间延长逐渐减弱。研究结果显示，饲料中添加灰树花多糖可在一定程度上增强吉富罗非鱼的非特异性免疫力。
위탐구회수화다당대길부라비어면역세포화항양화매활성적영향，재사료중첨가불동질량분수적회수화다당。장규격상동、질량140～150 g적길부라비어수궤분위3개처리조，사양재장유온도위26～30℃적폭기자래수적수상（60 cm ×42 cm ×38 cm ）중，투위첨가질량분수위0、0．05％、0．20％회수화다당적사료，사위일개월。시험결과표명，회수화다당능증가길부라비어홍세포화백세포수량，단대백세포탄서활성무명현영향。회수화다당대혈청、간장중과양화물매활성유현저촉진작용，차표현출명현적제량효응；대초양화물기화매활성표현출일정적저촉고억현상；과양화경매활성수다당적촉진작용수시간연장축점감약。연구결과현시，사료중첨가회수화다당가재일정정도상증강길부라비어적비특이성면역력。
In this experiment ,the effects of different concentrations of Gri f lola f rondosas polysaccharides (GFP) on the immunocytes and antioxidant enzyme activity were investigated in Nile tilapia Oreochromis niloticus .The tilapia with body weight of 140~150 g were randomly stocked into tanks of 60 cm × 42 cm × 38 cm ,and fed three kinds of feed supplemented with the polysaccharides at a rate of 0 ,0 .05% and 0 .20% with triplication at water temperature of 26~30 ℃ for one month .It was found that the GFP led to increase in the number of erythrocyte and leukocyte ,but had no significant effect on the phagocytic activity of leukocyte .The significant promotive and dose effect of GFP on the activity of peroxidase (POD) were observed in the serum and liver .The activity of superoxide dismutase (SOD) was induced by low concentration of GFP and inhibited by high concentration of GFP .The promotive effect of GFP on the activity of catalase (CAT ) was gradually decreased , indicating that GFP improves the non-specific immunity of tilapia to some extent .