CAJ | 학술논문

目的研究没药甾酮对肝星状细胞增殖的影响。方法采用MTT法观察不同浓度没药甾酮对肝星状细胞增殖的影响；应用流式细胞术检测细胞周期的变化；化学发光法检测细胞培养液中超氧化物歧化酶（SOD ）、丙二醛（MDA）、还原型谷胱甘肽（GSH）和过氧化氢酶（CAT）水平。结果没药甾酮可显著抑制肝星状细胞生长，并呈时间、剂量依赖，24 h最大抑制率可达（57．4±2．45）％（100μmol/L ）；50μmol/L和75μmol/L没药甾酮可使G2/M 期细胞比例增多，G0/G1和S期比例下降；没药甾酮可使MDA水平下降，GSH和SOD、CAT活性升高。结论没药甾酮可干扰细胞周期，抑制肝星状细胞增殖；还可降低氧化应激反应从而抑制肝星状细胞活化。
목적연구몰약치동대간성상세포증식적영향。방법채용MTT법관찰불동농도몰약치동대간성상세포증식적영향；응용류식세포술검측세포주기적변화；화학발광법검측세포배양액중초양화물기화매（SOD ）、병이철（MDA）、환원형곡광감태（GSH）화과양화경매（CAT）수평。결과몰약치동가현저억제간성상세포생장，병정시간、제량의뢰，24 h최대억제솔가체（57．4±2．45）％（100μmol/L ）；50μmol/L화75μmol/L몰약치동가사G2/M 기세포비례증다，G0/G1화S기비례하강；몰약치동가사MDA수평하강，GSH화SOD、CAT활성승고。결론몰약치동가간우세포주기，억제간성상세포증식；환가강저양화응격반응종이억제간성상세포활화。
Objective To investigate the effects of guggulsterone on cell proliferation of hepatic stellate cell line (HSC-T6) cultured in vitro .Methods HSC-T6 cells were treated with different concentrations of guggulsterone (5-100 μmol/L) for 24-72 hours .Cell proliferation was tested by MTT assay .Cell cycle was investigated using flow cytometer .Superoxide dismutase(SOD) ,malondialdehyde(MDA) ,glutathione(GSH) and catalas(CAT) levels in the culture media were measured by spectrophotometric colorimetry . Results Guggulsterone significantly inhibited HSC-T6 cell proliferation in a dose-dependent and time-dependent manner ,and the maximal inhibition effect was observed at the concentration of 100 μmol/L guggulsterone (57 .4% ± 2 .45% ) in 24 hours .Flow cytometer analysis showed that guggulsterone significantly enhanced G2/M but inhibited G0/G1 and S proportion of cell cycle in response to the treatment of 50 μmol/L and 75 μmol/L guggul-sterone respectively .Further analysis indicated that 50 μmol/L and 75 μmol/L guggulsterone significantly decreased the level of MDA but increased the activity of GSH and levels of SOD and CAT in the culture medium .Conclusion Gugguls-terone can inhibit cell proliferation through interfering with cell cycle in hepatic stellate cells .Moreover ,guggulsterone can reduce ocidative stress to inhibit the activation of hepatic stellate cells .Therefore ,both of these effects induced by gugguls-terone will contribute to the inhibition of liver fibrosis .