CAJ | 학술논문

目的研究不同肝癌细胞对干扰素（IFN-γ）的敏感性是否存在差异。方法定量RT-PCR检测9种肝癌细胞系的IFN-γ受体表达，并通过比较IFN-γ处理前后 HepG2、SNU182、SNU449和 PLC 4种细胞的周期变化和细胞凋亡情况，判定不同 HCC细胞系对IFN-γ的敏感性差异。结果 HepG2、SNU449细胞IFN-γ受体IFNGR1表达低于正常肝组织（＜0．5），Huh-7的 IFNGR1和 IFNGR2的表达均低于正常肝组织（＜0．5）。IFN-γ能够诱导 HepG2、SNU182和SNU449细胞凋亡，并能阻滞 HepG2细胞的G1期向S期的细胞周期转换，SNU182细胞的G2/M 期转换，但对PLC细胞生长抑制作用不明显。结论 IFN-γ可通过促进细胞凋亡和细胞周期阻滞抑制肝癌细胞生长，不同的肝癌细胞对IFN-γ敏感性存在差异，提示临床应用IFN-γ进行肝癌治疗时应考虑个体化差异。
목적연구불동간암세포대간우소（IFN-γ）적민감성시부존재차이。방법정량RT-PCR검측9충간암세포계적IFN-γ수체표체，병통과비교IFN-γ처리전후 HepG2、SNU182、SNU449화 PLC 4충세포적주기변화화세포조망정황，판정불동 HCC세포계대IFN-γ적민감성차이。결과 HepG2、SNU449세포IFN-γ수체IFNGR1표체저우정상간조직（＜0．5），Huh-7적 IFNGR1화 IFNGR2적표체균저우정상간조직（＜0．5）。IFN-γ능구유도 HepG2、SNU182화SNU449세포조망，병능조체 HepG2세포적G1기향S기적세포주기전환，SNU182세포적G2/M 기전환，단대PLC세포생장억제작용불명현。결론 IFN-γ가통과촉진세포조망화세포주기조체억제간암세포생장，불동적간암세포대IFN-γ민감성존재차이，제시림상응용IFN-γ진행간암치료시응고필개체화차이。
Objective To investigate the diversity of different hepatocellular carcinoma (HCC) cell lines in response to IFN-γ treatment in vitro .Methods Expression of receptors IFN-γin 9 kinds of HCC cell lines was detected using real-time (RT)-PCR .After IFN-γ was treated ,the changes of cell cycle and proportion of apoptotic cell in HepG2、SNU182、SNU449 and PLC cells were measured by flow cytometry .Results Low expression of IFNGR1 was observed in HepG2、SNU449 and Huh-7 cell lines ,low expression of IFNGR2 also exhibited in Huh-7 cell line(< 0 .5) ,when compared to normal liver tissues .IFN-γ treatment induced apoptosis of HepG2 ,SNU182 and SNU449 cell ,cause cycle retardation in G1-S phase transition of HepG2 cell and G2/M arrest of SNU182 cell .No obvious inhibitory effects on PLC cell were observed .Conclusion IFN-γcould inhibit the proliferation of HCC by promoting cell apoptosis and cell cycle arrest .There are differences in HCC cells sensitivity to IFN-γ.In clinical practice ,individual differences should be considered while HCC was treated with IFN-γ.