现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
12期
1270-1272,1275
,共4页
燕忠生%魏千程%李俊%石鲲%李世昌%田种泽
燕忠生%魏韆程%李俊%石鯤%李世昌%田種澤
연충생%위천정%리준%석곤%리세창%전충택
痰热清注射液%放射性肺炎%IL-6%TNF-α%TGF-β1%NF-κB
痰熱清註射液%放射性肺炎%IL-6%TNF-α%TGF-β1%NF-κB
담열청주사액%방사성폐염%IL-6%TNF-α%TGF-β1%NF-κB
Tanreqing injection%radiation pneumonitis%IL-6%TNF-α%TGF-β1%NF-κB
目的：探讨痰热清注射液防治放射性肺炎的作用及其机制。方法40只雌性SD大鼠随机分为正常组、痰热清组、地塞米松组和模型组各10只，除正常组外其余各组大鼠均用直线加速器对大鼠双肺进行单次照射25 Gy造模。造模后第1天起模型组和正常组予生理盐水灌胃，其余2组分别用痰热清、地塞米松灌胃，连续6周后处死，观察各组大鼠肺系数及肺组织病理改变，检测血清IL-6、TNF-α、TGF-β1的水平和肺组织中 P65核转位水平及磷酸化IκB的表达。结果痰热清组及地塞米松组肺系数、肺组织炎性及纤维化改变、血清IL-6、TNF-α、TGF -β1水平均显著低于模型组（P均＜0．01），且上述各指标痰热清组均优于地塞米松组（ P均＜0．01）；痰热清组和地塞米松组细胞核 P65和磷酸化IκB的表达均显著低于模型组（P均＜0．01）。结论痰热清注射液可能通过抑制P65的核转位及IκB的磷酸化，抑制血清中IL-6、TNF-α、TGF-β1的合成，从而对大鼠放射性肺炎起到防治作用，反向验证了热毒为放射性肺炎的主要病机之一。
目的：探討痰熱清註射液防治放射性肺炎的作用及其機製。方法40隻雌性SD大鼠隨機分為正常組、痰熱清組、地塞米鬆組和模型組各10隻，除正常組外其餘各組大鼠均用直線加速器對大鼠雙肺進行單次照射25 Gy造模。造模後第1天起模型組和正常組予生理鹽水灌胃，其餘2組分彆用痰熱清、地塞米鬆灌胃，連續6週後處死，觀察各組大鼠肺繫數及肺組織病理改變，檢測血清IL-6、TNF-α、TGF-β1的水平和肺組織中 P65覈轉位水平及燐痠化IκB的錶達。結果痰熱清組及地塞米鬆組肺繫數、肺組織炎性及纖維化改變、血清IL-6、TNF-α、TGF -β1水平均顯著低于模型組（P均＜0．01），且上述各指標痰熱清組均優于地塞米鬆組（ P均＜0．01）；痰熱清組和地塞米鬆組細胞覈 P65和燐痠化IκB的錶達均顯著低于模型組（P均＜0．01）。結論痰熱清註射液可能通過抑製P65的覈轉位及IκB的燐痠化，抑製血清中IL-6、TNF-α、TGF-β1的閤成，從而對大鼠放射性肺炎起到防治作用，反嚮驗證瞭熱毒為放射性肺炎的主要病機之一。
목적：탐토담열청주사액방치방사성폐염적작용급기궤제。방법40지자성SD대서수궤분위정상조、담열청조、지새미송조화모형조각10지，제정상조외기여각조대서균용직선가속기대대서쌍폐진행단차조사25 Gy조모。조모후제1천기모형조화정상조여생리염수관위，기여2조분별용담열청、지새미송관위，련속6주후처사，관찰각조대서폐계수급폐조직병리개변，검측혈청IL-6、TNF-α、TGF-β1적수평화폐조직중 P65핵전위수평급린산화IκB적표체。결과담열청조급지새미송조폐계수、폐조직염성급섬유화개변、혈청IL-6、TNF-α、TGF -β1수평균현저저우모형조（P균＜0．01），차상술각지표담열청조균우우지새미송조（ P균＜0．01）；담열청조화지새미송조세포핵 P65화린산화IκB적표체균현저저우모형조（P균＜0．01）。결론담열청주사액가능통과억제P65적핵전위급IκB적린산화，억제혈청중IL-6、TNF-α、TGF-β1적합성，종이대대서방사성폐염기도방치작용，반향험증료열독위방사성폐염적주요병궤지일。
Objective It is to approach the effect of Tanreqing injection in inhibit radiation pneumonitis and explore its mechanism .Methods 40 healthy SD rats were divided into normal group , irradiation with Tanreqing group , dexamethasone group and model group, 10 cases in each group.The animals were given corresponding drugs by gavage treatment for 6 weeks. The determination of lung coefficient , pulmonary fibrosis score and pathological changes of lung with Hematoxylin and eosin ( HE) staining were explored to observe the level of radiation pneumonitis .Enzyme-linked immunosorbent assay ( ELISA) was applied to detect the plasma interleukin -6 (IL-6), necrosis factor-α(TNF-α) and transforming growth factor β1 (TGF-β1 ) levels.Western-Blot was applied to detect the translocation of P 65.And in order to investigate the molecular basis of the altered subcellular localization of P 65, phospho-IκBαand total IκBαprotein was tested still by Western-blot method.Re-sults The lung coefficient, pulmonary fibrosis score and the expression of IL -6, TNF-αand TGF-β1 inTanreqing group and dexamethasone group were remarkably lower than that in model group (all P<0.01), and these indexes in Tanreqing group were significantly lower than that in dexamethasone group (all P<0.01).The expression of P65 in nucler and phospho-IκBαin in Tanreqing group and dexamethasone group was remarkable higher than that of the model groups ( all P<0.01 ) . Conclusion Tanreqing injgection can inhibit radiation pneumonitis , whose mechanism probably related to downregulation the expression of P65 in nucler and phospho -IκBα, and represses the expression of IL -6 , TNF-αand TGF-β1 , reverse proved that deficiency of Re and Du is one of traditional Chinese medicine main pathogenesis in radiation pneumonitis .
