国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2014年
3期
172-175
,共4页
肝炎病毒,乙型%肝细胞癌%X/preC区%基因突变
肝炎病毒,乙型%肝細胞癌%X/preC區%基因突變
간염병독,을형%간세포암%X/preC구%기인돌변
Hepatitis B virus%Hepatocellular carcinoma%X/preC region%Gene mutation
目的 探讨HBV C基因型X/preC区基因突变与HCC的相关性.方法 收集2010年9月至2013年6月就诊于温州市中医院的HCC患者和非HCC慢性HBV感染者的外周血样本,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)法检测病毒基因型,将确认为C型的病例血清样本进行基因测序检测X/preC区的突变情况.结果 HCC患者中X/preC区单碱基位点突变频率从高到低依次为G1764A(97.67％)、A1762T (95.35％)、G1896A (80.23％)、T1753V (59.30％)、A1846T (55.81％)、C1653T (44.18％)、G 1613A (40.67％)和G1899A(32.59％),且各位点的突变频率均高于非HCC组,差异均具有统计学意义(x2=23.604、22.956、14.902、34.318、26.078、10.623、6.137和13.464,P均＜0.05).分析两组患者X/preC区累计突变的数目发现,HCC患者X/preC区累积碱基突变个数≥4个的比例为88.37％ (76/86),而非HCC患者X/preC区累积碱基突变个数≥4个的比例为31.48％(34/108),两组比较差异具有统计学意义(x2=63.122,P＜0.01).结论 HBV相关的HCC患者X/preC区基因突变率较非HCC患者高,且HCC患者更容易发生X/preC基因的多位点突变.
目的 探討HBV C基因型X/preC區基因突變與HCC的相關性.方法 收集2010年9月至2013年6月就診于溫州市中醫院的HCC患者和非HCC慢性HBV感染者的外週血樣本,採用限製性片段長度多態性聚閤酶鏈反應(PCR-RFLP)法檢測病毒基因型,將確認為C型的病例血清樣本進行基因測序檢測X/preC區的突變情況.結果 HCC患者中X/preC區單堿基位點突變頻率從高到低依次為G1764A(97.67％)、A1762T (95.35％)、G1896A (80.23％)、T1753V (59.30％)、A1846T (55.81％)、C1653T (44.18％)、G 1613A (40.67％)和G1899A(32.59％),且各位點的突變頻率均高于非HCC組,差異均具有統計學意義(x2=23.604、22.956、14.902、34.318、26.078、10.623、6.137和13.464,P均＜0.05).分析兩組患者X/preC區纍計突變的數目髮現,HCC患者X/preC區纍積堿基突變箇數≥4箇的比例為88.37％ (76/86),而非HCC患者X/preC區纍積堿基突變箇數≥4箇的比例為31.48％(34/108),兩組比較差異具有統計學意義(x2=63.122,P＜0.01).結論 HBV相關的HCC患者X/preC區基因突變率較非HCC患者高,且HCC患者更容易髮生X/preC基因的多位點突變.
목적 탐토HBV C기인형X/preC구기인돌변여HCC적상관성.방법 수집2010년9월지2013년6월취진우온주시중의원적HCC환자화비HCC만성HBV감염자적외주혈양본,채용한제성편단장도다태성취합매련반응(PCR-RFLP)법검측병독기인형,장학인위C형적병례혈청양본진행기인측서검측X/preC구적돌변정황.결과 HCC환자중X/preC구단감기위점돌변빈솔종고도저의차위G1764A(97.67％)、A1762T (95.35％)、G1896A (80.23％)、T1753V (59.30％)、A1846T (55.81％)、C1653T (44.18％)、G 1613A (40.67％)화G1899A(32.59％),차각위점적돌변빈솔균고우비HCC조,차이균구유통계학의의(x2=23.604、22.956、14.902、34.318、26.078、10.623、6.137화13.464,P균＜0.05).분석량조환자X/preC구루계돌변적수목발현,HCC환자X/preC구루적감기돌변개수≥4개적비례위88.37％ (76/86),이비HCC환자X/preC구루적감기돌변개수≥4개적비례위31.48％(34/108),량조비교차이구유통계학의의(x2=63.122,P＜0.01).결론 HBV상관적HCC환자X/preC구기인돌변솔교비HCC환자고,차HCC환자경용역발생X/preC기인적다위점돌변.
Objective To explore the correlation between X/preC region mutations in HBV genotype C and HCC.Methods Serum samples from patients with HCC and chronic HBV infection (non-HCC) were collected in Wenzhou Chinese Medicine Hospital from September 2010 to June 2013.HBV genotypes were determined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP),and then the samples of genotype C HBV were sequenced for the mutations in X/preC region.Results The single base mutation frequencies in X/preC region in HCC patients were G1764A (97.67％),A1762T (95.35％),G1896A (80.23％),T1753V (59.30％),A1846T(55.81％),C1653T(44.18％),G1613A(40.67％) and G1899A(32.59％) in order,which were all higher than those in non-HCC patients with significant differences (x2=23.604,22.956,14.902,34.318,26.078,10.623,6.137 and 13.464,P all＜0.05).Moreover,the proportion of HCC patients with multi-mutation (the total number of mutations ≥4 in X/preC region) was 88.37％(76/86),which was higher than that of non-HCC patients [31.48％(34/108)],with a significant difference(x2=63.122,P＜0.01).Conclusions HBV related HCC patients have higher gene mutation frequency and multiple mutational sites in X/preC region than non-HCC patients do.
