中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2420-2422
,共3页
杨扬%张敏%任晓静%王琦%武希润%申慧琴
楊颺%張敏%任曉靜%王琦%武希潤%申慧琴
양양%장민%임효정%왕기%무희윤%신혜금
肿瘤易感基因101%血管形成%血管内皮生长因子%胃癌
腫瘤易感基因101%血管形成%血管內皮生長因子%胃癌
종류역감기인101%혈관형성%혈관내피생장인자%위암
Tumor susceptibility gene 101%Angiogenesis%Vascular endothelial growth factor%Gastric cancer
目的 观察肿瘤易感基因101(TSG101)对胃癌细胞血管形成的影响.方法 对不同TSG101表达水平的3组胃癌细胞亚系,分别进行体内实验和体外实验,检测血管内皮生长因子(VEGF)及其受体(VEGFR)的表达及血管形成.结果 (1)体外实验:TSG101组细胞促人脐静脉内皮细胞(HUVEC)成管数目明显多于空质粒组与未转染组[(19.50±3.02)个比(11.50±2.51)、(11.34±1.63)个,P＜0.05],TSG101组细胞VEGF分泌水平高于空质粒组与未转染组[(514.80±18.16) ng/L比(306.58±20.86)、(305.69±29.93) ng/L,P＜0.05];(2)体内实验:TSG101组荷瘤裸鼠瘤组织微血管密度和血清VEGF水平均明显高于空质粒组与未转染组[11.16±2.31比5.33±1.63、4.66±1.36,(340.64±19.15) ng/L比(219.34±13.56)、(206.10±25.85) ng/L,P＜0.05].TSG101组荷瘤裸鼠瘤组织中VEGF、VEGFR表达(0.548 ±0.032、0.345±0.042)明显高于未转染组(0.350±0.034、0.203±0.030)与空质粒组(0.327±0.020、0.206±0.036,P＜ 0.05).结论 TSG101高表达的SGC7901细胞可能通过上调VEGF的表达促进胃癌血管形成.
目的 觀察腫瘤易感基因101(TSG101)對胃癌細胞血管形成的影響.方法 對不同TSG101錶達水平的3組胃癌細胞亞繫,分彆進行體內實驗和體外實驗,檢測血管內皮生長因子(VEGF)及其受體(VEGFR)的錶達及血管形成.結果 (1)體外實驗:TSG101組細胞促人臍靜脈內皮細胞(HUVEC)成管數目明顯多于空質粒組與未轉染組[(19.50±3.02)箇比(11.50±2.51)、(11.34±1.63)箇,P＜0.05],TSG101組細胞VEGF分泌水平高于空質粒組與未轉染組[(514.80±18.16) ng/L比(306.58±20.86)、(305.69±29.93) ng/L,P＜0.05];(2)體內實驗:TSG101組荷瘤裸鼠瘤組織微血管密度和血清VEGF水平均明顯高于空質粒組與未轉染組[11.16±2.31比5.33±1.63、4.66±1.36,(340.64±19.15) ng/L比(219.34±13.56)、(206.10±25.85) ng/L,P＜0.05].TSG101組荷瘤裸鼠瘤組織中VEGF、VEGFR錶達(0.548 ±0.032、0.345±0.042)明顯高于未轉染組(0.350±0.034、0.203±0.030)與空質粒組(0.327±0.020、0.206±0.036,P＜ 0.05).結論 TSG101高錶達的SGC7901細胞可能通過上調VEGF的錶達促進胃癌血管形成.
목적 관찰종류역감기인101(TSG101)대위암세포혈관형성적영향.방법 대불동TSG101표체수평적3조위암세포아계,분별진행체내실험화체외실험,검측혈관내피생장인자(VEGF)급기수체(VEGFR)적표체급혈관형성.결과 (1)체외실험:TSG101조세포촉인제정맥내피세포(HUVEC)성관수목명현다우공질립조여미전염조[(19.50±3.02)개비(11.50±2.51)、(11.34±1.63)개,P＜0.05],TSG101조세포VEGF분비수평고우공질립조여미전염조[(514.80±18.16) ng/L비(306.58±20.86)、(305.69±29.93) ng/L,P＜0.05];(2)체내실험:TSG101조하류라서류조직미혈관밀도화혈청VEGF수평균명현고우공질립조여미전염조[11.16±2.31비5.33±1.63、4.66±1.36,(340.64±19.15) ng/L비(219.34±13.56)、(206.10±25.85) ng/L,P＜0.05].TSG101조하류라서류조직중VEGF、VEGFR표체(0.548 ±0.032、0.345±0.042)명현고우미전염조(0.350±0.034、0.203±0.030)여공질립조(0.327±0.020、0.206±0.036,P＜ 0.05).결론 TSG101고표체적SGC7901세포가능통과상조VEGF적표체촉진위암혈관형성.
Objective To investigate the effect of tumor susceptibility gene 101 (TSG101) on angiogenesis of gastric cancer SGC7901 cells.Methods Cells were divided into the non-transfection group,the empty plasmid transfection group and TSG101 eukaryotic expression plasmid transfection group (the TSG101 transfection group).Then we examined the relationship between TSG101 expression and tumor angiogenesis in vivo and in vitro experiment respectively.To detect the expression of vascular endothelial growth factor (VEGF),vascular endothelial growth factor receptor (VEGFR) and the angiogenesis.Resuits (1) It was showed that the angiogenesis number of HUVEC and the expression levels of VEGF in the TSG101 transfection group was significantly higher than that of the empty plasmid transfection group and the non-transfection group in vitro experiment (19.50 ± 3.02 vs.11.50 ± 2.51,11.34 ± 1.63 and 514.80 ± 18.16 vs.306.58 ± 20.86,305.69 ± 29.93,all P ＜ 0.05).(2) It was showed the microvascular density in tumor tissue of nude mice and the serum VEGF concentration of the TSG101 transfection group was significantly higher than that of the empty plasmid transfection group and the non-transfection group in vivo experiment [11.16±2.31 vs.5.33 ±1.63,4.66 ±1.36 and (340.64±19.15) ng/L vs.(219.34 ± 13.56),(206.10 ± 25.85) ng/L,all P ＜ 0.05].The expressions of VEGF and VEGFR of the TSG101 transfection grou p (0.548 ± 0.032,0.345 ± 0.042) of tumor tissue in nude mice were significantly higher than that of the non-transfection group (0.350 ± 0.034,0.203 ± 0.030) and the empty plasmid transfection group (0.327 ± 0.020,0.206 ± 0.036,P ＜ 0.05).Conclusion The high-expression of TSG101 in SGC7901 cells may promote angiogenesis of gastric cancer by up-regulated expression of VEGF.
