CAJ | 학술논문

目的：明确人源电压门控质子通道蛋白（human voltage-gated proton channel 1，Hv1）对乳腺癌细胞迁移及侵袭能力的影响。方法：检测Hv1在不同转移能力的人乳腺癌细胞系中的表达，利用小RNA干扰（siRNA）技术下调Hv1在乳腺癌MDA-MB-231细胞中的表达，通过细胞划痕和体外侵袭实验方法，观察Hv1对乳腺癌细胞迁移和侵袭的影响并初步探讨相关分子机制。结果：Hv1在高转移的乳腺癌细胞MDA-MB-231中表达较高，Hv1基因的siRNA干扰片段能够抑制Hv1基因及蛋白的表达；细胞划痕和体外侵袭实验表明Hv1降表达的MDA-MB-231细胞迁移和侵袭能力较弱；明胶酶谱和免疫印迹实验证明下调Hv1基因在MDA-MB-231细胞中的表达明显抑制了MMP-2的活性。结论：Hv1能够促进乳腺癌细胞迁移及侵袭。
목적：명학인원전압문공질자통도단백（human voltage-gated proton channel 1，Hv1）대유선암세포천이급침습능력적영향。방법：검측Hv1재불동전이능력적인유선암세포계중적표체，이용소RNA간우（siRNA）기술하조Hv1재유선암MDA-MB-231세포중적표체，통과세포화흔화체외침습실험방법，관찰Hv1대유선암세포천이화침습적영향병초보탐토상관분자궤제。결과：Hv1재고전이적유선암세포MDA-MB-231중표체교고，Hv1기인적siRNA간우편단능구억제Hv1기인급단백적표체；세포화흔화체외침습실험표명Hv1강표체적MDA-MB-231세포천이화침습능력교약；명효매보화면역인적실험증명하조Hv1기인재MDA-MB-231세포중적표체명현억제료MMP-2적활성。결론：Hv1능구촉진유선암세포천이급침습。
Objective:To clarify the effect of voltage-gated proton channel 1 (Hv1) on the migration and invasion of breast cancer cells. Methods:The protein expression of Hv1 was detected in human breast cancer cell lines with different metastatic abilities. SiRNA technique was used to down-regulate the expression of Hvl in breast cancer MDA-MB-231 cells. Scratch and matrigel invasion methods were used to observe the effect of Hvl on the migration and invasion of breast cancer cells, and the relevant molecular mechanism was explored. Results:Hv1 was highly expressed in the highly metastatic breast cancer cell line MDA-MB-231. Hvl was more highly expressed in MDA-MB-231 cells with higher metastatic ability. The SiRNA sequence target at Hvl inhibited Hvl expression. Scratch and matrigel invasion experiments showed that the migration and invasion of MDA-MB-231 cells were significantly attenuated when Hv1 was knocked down by siRNA targeting Hv1. Zymography experiment on matrix metalloproteinase indicated that the enzyme activities of MMP-2 markedly decreased. Conclusion:Hv1 promoted the migration and invasion ability of breast cancer cells.