CAJ | 학술논문

目的探讨补阳还五汤对局灶性脑缺血大鼠BrdU及vWF的影响。方法采用大脑中动脉线栓法建立右侧局灶性脑缺血大鼠模型,将动物随机分为正常组、假手术组、模型组、补阳还五汤组,腹腔注射Brdu,分别于1d、7d、14d和28d处死动物,采用免疫组织化学法观察 BrdU及 vWF的表达。结果正常脑内存在一定的BrdU及vWF阳性细胞,脑缺血后表达迅速增加,7d达高峰,然后逐渐下降；补阳还五汤可显著增强 BrdU及 vWF的表达,在第7d、14d时明显多于其它各组（P<0.05）。结论补阳还五汤可促进局灶性脑缺血大鼠缺血区BrdU及vWF的表达。
목적탐토보양환오탕대국조성뇌결혈대서BrdU급vWF적영향。방법채용대뇌중동맥선전법건립우측국조성뇌결혈대서모형,장동물수궤분위정상조、가수술조、모형조、보양환오탕조,복강주사Brdu,분별우1d、7d、14d화28d처사동물,채용면역조직화학법관찰 BrdU급 vWF적표체。결과정상뇌내존재일정적BrdU급vWF양성세포,뇌결혈후표체신속증가,7d체고봉,연후축점하강；보양환오탕가현저증강 BrdU급 vWF적표체,재제7d、14d시명현다우기타각조（P<0.05）。결론보양환오탕가촉진국조성뇌결혈대서결혈구BrdU급vWF적표체。
Objective:To explore the ef ect of Buyang Huanwu Decoction (BYHWD)on BrdU and vWF in rats after focal cerebral ischemia. Methods:The model of focal cerebral ischemia in rats was reproduced by middle cerebral artery occlusion.Rats were randomly divided into fol owing groups including navie,shame-operated,ischemia and Buyang Huanwu Decoction (BYHWD) groups. Animals were received BrdU-labeled and kil ed at 1,7,14 and 28 day point after operation.BrdU and vWF were measured by immunohistochemical staining. Result:There was a certain BrdU or vWF positive cel s in normal brain, after cerebral ischemia BrdU and vWF positive cel s were increased and reached the peak at 7 day. BYHWD can significantly increase the expression of BrdU or vWF. The increase was statistical y significant at 7 and 14 day point after ischemia(P<0.05).Compared to the other groups,there was statistical y significant on BYHWD for decreasing the number of nerve function at 7 and 14 day points(P<0.05). Conclusion:BYHWD may promote the expression of BrdU and vWF in rats after focal cerebral ischemia.