CAJ | 학술논문

目的了解四种不同厂家生产的非梅毒螺旋体抗原血清学试验试剂检测结果的一致性。方法分别用一种梅毒快速血浆反应素（RPR）试剂和三种甲苯胺红血清不需加热试验(TRUST)试剂对收集的280份血清做盲法筛检。用χ2检验和相关样本非参数检验法对检测的定性和定量结果作统计学分析。结果四种试剂定性检测结果无统计学差异（χ2=2.63，P>0.05），但定量分析结果存在1~2个滴度的差异，且该差异具有统计学意义（χ2=24.22，P<0.001）。结论用非梅毒螺旋体抗原血清试验进行检测时，应注意不同试剂检测结果在定量时可能存在一定差异，在梅毒的监测和疗效评估时应注意不同试剂产生的检验误差。
목적료해사충불동엄가생산적비매독라선체항원혈청학시험시제검측결과적일치성。방법분별용일충매독쾌속혈장반응소（RPR）시제화삼충갑분알홍혈청불수가열시험(TRUST)시제대수집적280빈혈청주맹법사검。용χ2검험화상관양본비삼수검험법대검측적정성화정량결과작통계학분석。결과사충시제정성검측결과무통계학차이（χ2=2.63，P>0.05），단정량분석결과존재1~2개적도적차이，차해차이구유통계학의의（χ2=24.22，P<0.001）。결론용비매독라선체항원혈청시험진행검측시，응주의불동시제검측결과재정량시가능존재일정차이，재매독적감측화료효평고시응주의불동시제산생적검험오차。
Objective To know the consistency of the results of four reagents for non-treponemal antigen serological test from dif erent manufacturers. Methods 280 samples were col ected and tested with a syphilis rapid plasma reagain (RPR)and three toluidine red unheated serum test reagents (TRUST). Qualitative and quantitative results were tested byχ2test and related-sample non-parametric test. Results There were no significant dif erence in qualitative results (χ2=2.63,P>0.05), but1-2units of dif erence in titer in quantitative results,and the dif erence was statistical y significant (χ2=24.22,P<0.001). Conclusion The dif erence in quantitative results of dif erent reagents should be paid at ention to in Non-treponemal antigen tests,and the dif erence in dif erent reagents should be paid at ention to in monitoring and evaluating the ef icacy of treating.