CAJ | 학술논문

目的探讨介入围手术期干扰素-α（IFN-α）联合全反式维甲酸（ATRA）治疗肝细胞癌的疗效。方法选取28只Wistar大鼠，建立肝癌细胞CBRH7919的动物模型，并将瘤块组织移植于大鼠体内。1个月后，待瘤块完全形成后将所有大鼠的肝动脉进行结扎，并将所有大鼠随机分为实验组与对照组，各14只。对照组给予腹腔注射生理盐水，实验组给予腹腔注射IFN-α联合ATRA进行治疗。10d后将所有大鼠处死，并检测肝癌组织中血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bF-GF）mRNA及蛋白的表达水平、癌细胞的凋亡情况与瘤体内的微血管密度。结果实验组外周血VEGF水平、肿瘤微血管密度及肿瘤细胞凋亡指数[（91．8±12．7）pg/mL，（115．7±11．8）个/HP，（12．29±4．53）％]均明显低于对照组[（67．4±18．3）pg/mL，（65．3±7．2）个/HP，（3．81±1．52）％]，差异有统计学意义（P＜0．05）；逆转录-聚合酶链反应（RT-PCR）及免疫组织化学法检测的结果均显示，实验组bFGF及VEGF表达要明显低于对照组。结论围介入手术期IFN-α联合ATRA的使用能够抑制新生肿瘤血管的生成，提高介入手术的临床疗效，值得推广。
목적탐토개입위수술기간우소-α（IFN-α）연합전반식유갑산（ATRA）치료간세포암적료효。방법선취28지Wistar대서，건립간암세포CBRH7919적동물모형，병장류괴조직이식우대서체내。1개월후，대류괴완전형성후장소유대서적간동맥진행결찰，병장소유대서수궤분위실험조여대조조，각14지。대조조급여복강주사생리염수，실험조급여복강주사IFN-α연합ATRA진행치료。10d후장소유대서처사，병검측간암조직중혈관내피생장인자（VEGF）、감성성섬유세포생장인자（bF-GF）mRNA급단백적표체수평、암세포적조망정황여류체내적미혈관밀도。결과실험조외주혈VEGF수평、종류미혈관밀도급종류세포조망지수[（91．8±12．7）pg/mL，（115．7±11．8）개/HP，（12．29±4．53）％]균명현저우대조조[（67．4±18．3）pg/mL，（65．3±7．2）개/HP，（3．81±1．52）％]，차이유통계학의의（P＜0．05）；역전록-취합매련반응（RT-PCR）급면역조직화학법검측적결과균현시，실험조bFGF급VEGF표체요명현저우대조조。결론위개입수술기IFN-α연합ATRA적사용능구억제신생종류혈관적생성，제고개입수술적림상료효，치득추엄。
Objective To study the effect of IFN-αcombined with ATRA in liver cancer therapy in perioperative phase of inter-vention .Methods An animal model of liver cancer CBRH7919 was established by implanting 28 Wistar rats with tumor tissue .The hepatic artery was tied off in one month after formation of tumor .All the rats were randomly divided into the experimental group (14 cases) and the control group(14 cases) .The control group accepted peritoneal injection with normal saline while the experimen-tal group accepted IFN-αcombined with ATRA .10 days later ,all the rats were sacrificed .And the level of expression of VEGF ,bF-GF ,mirovessel density ,and apoptotic index of tumor cells were measured .Results Serum VEGF level ,MVD and apoptotic index of tumor cells of the experimental group were statistically lower than that of the control group [(91 .8 ± 12 .7) pg/mL ,(115 .7 ± 11 .8)/HP ,(12 .29 ± 4 .53)% vs .(67 .4 ± 18 .3)pg/mL ,(65 .3 ± 7 .2)/HP ,(3 .81 ± 1 .52)% ,t=4 .099、13 .642、6 .640 ,P<0 .05)] , results of RT-PCR and immunohistochemistry detection both showed that the levels of expression of VEGF and bFGF of the exper-imental group was lower than that of the control group .Conclusion IFN-αcombined with ATRA in liver cancer therapy in periop-erative phase of intervention could suppress the generation of neoplastic vascular and improve the effect of the intervention .So it is worth promoting .