CAJ | 학술논문

目的探讨简单、实用、有效的棘阿米巴角膜炎临床与实验室诊断方法。方法观察本院5例棘阿米巴角膜炎感染的临床表现，通过湿封片或染色后镜检角膜刮片、分离培养棘阿米巴、行角膜组织病理学检查，分析棘阿米巴角膜炎的临床思维。结果角膜刮片显微镜下观察到棘阿米巴包囊、滋养体和伪足，分离培养和组织活检能检测到阿米巴，5例患者棘阿米巴的诊断能得到确诊。结论怀疑棘阿米巴角膜炎病例，应尽早行棘阿米巴病原学检测，对于棘阿米巴的早期诊断，防止误诊、早期治疗和预后有重要意义。
목적탐토간단、실용、유효적극아미파각막염림상여실험실진단방법。방법관찰본원5례극아미파각막염감염적림상표현，통과습봉편혹염색후경검각막괄편、분리배양극아미파、행각막조직병이학검사，분석극아미파각막염적림상사유。결과각막괄편현미경하관찰도극아미파포낭、자양체화위족，분리배양화조직활검능검측도아미파，5례환자극아미파적진단능득도학진。결론부의극아미파각막염병례，응진조행극아미파병원학검측，대우극아미파적조기진단，방지오진、조기치료화예후유중요의의。
purpose To explore a simple, practical effective method for clinical diagnosing Acanthamoeba keratitis and laboratory identifing Acanthamoeba. Methods Observed about 5 cases of acanthamoeba keratitis infection clinical manifestations in our hospital, and examined corneal scraping by 10% potassium hydroxide(KOH) wet-mount or dyeing. At the same time, Acanthamoeba was isolated and cultured from the corneal tissue, which would be analyse clinical thinking of acanthamoeba keratitis. Results The cysts,Trophozoites and pseudopods the Trophozoites of Acanthamoeba were directly observed under the microscope. Acanthamoeba culture and Corneal histopathology are positive, so 5 cases of acanthamoeba diagnosis can be confirmed. Conclusion Suspected of cases of acanthamoeba keratitis, acanthamoeba etiology detection is needed as soon as possible, for the early diagnosis of acanthamoeba has important significance to prevent misdiagnosis, early treatment and prognosis.