中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
3期
1138-1142
,共5页
二异丙酚%脂多糖类%蛋白质组学
二異丙酚%脂多糖類%蛋白質組學
이이병분%지다당류%단백질조학
Propofol%Lipopolysaccharides%Proteomics
目的观察异丙酚对脂多糖（LPS）所致人单核细胞（THP1）损伤的蛋白质表达谱变化，探讨其可能的作用机制。方法将体外培养的THP1细胞分为对照组，1μg/ml LPS刺激组（ L组），1μg/ml LPS加50μmol/L异丙酚组（ L＋P组），双向电泳分离三组THP1细胞中全部蛋白质，选择差异蛋白质点进行质谱分析，用Western blot对部分蛋白进行验证。结果经双向凝胶电泳和图像分析，发现9个蛋白点的表达水平在三组间有显著差异，经质谱分析得到4个蛋白质点的鉴定，即Stathmin 1、泛素-蛋白连接酶（ ubiquitin-conjugating enzyme E2N）、（Chain A，I113t Mutant Of Human Sod1）和程序性细胞死亡因子6（programmed cell death 6）。结论获得重复性和分辨率较好的THP1细胞双向凝胶电泳图谱，初步鉴定了部分与异丙酚保护LPS所致THP1细胞损伤作用相关的蛋白，为进一步了解异丙酚作用机制提供了新线索。
目的觀察異丙酚對脂多糖（LPS）所緻人單覈細胞（THP1）損傷的蛋白質錶達譜變化，探討其可能的作用機製。方法將體外培養的THP1細胞分為對照組，1μg/ml LPS刺激組（ L組），1μg/ml LPS加50μmol/L異丙酚組（ L＋P組），雙嚮電泳分離三組THP1細胞中全部蛋白質，選擇差異蛋白質點進行質譜分析，用Western blot對部分蛋白進行驗證。結果經雙嚮凝膠電泳和圖像分析，髮現9箇蛋白點的錶達水平在三組間有顯著差異，經質譜分析得到4箇蛋白質點的鑒定，即Stathmin 1、汎素-蛋白連接酶（ ubiquitin-conjugating enzyme E2N）、（Chain A，I113t Mutant Of Human Sod1）和程序性細胞死亡因子6（programmed cell death 6）。結論穫得重複性和分辨率較好的THP1細胞雙嚮凝膠電泳圖譜，初步鑒定瞭部分與異丙酚保護LPS所緻THP1細胞損傷作用相關的蛋白，為進一步瞭解異丙酚作用機製提供瞭新線索。
목적관찰이병분대지다당（LPS）소치인단핵세포（THP1）손상적단백질표체보변화，탐토기가능적작용궤제。방법장체외배양적THP1세포분위대조조，1μg/ml LPS자격조（ L조），1μg/ml LPS가50μmol/L이병분조（ L＋P조），쌍향전영분리삼조THP1세포중전부단백질，선택차이단백질점진행질보분석，용Western blot대부분단백진행험증。결과경쌍향응효전영화도상분석，발현9개단백점적표체수평재삼조간유현저차이，경질보분석득도4개단백질점적감정，즉Stathmin 1、범소-단백련접매（ ubiquitin-conjugating enzyme E2N）、（Chain A，I113t Mutant Of Human Sod1）화정서성세포사망인자6（programmed cell death 6）。결론획득중복성화분변솔교호적THP1세포쌍향응효전영도보，초보감정료부분여이병분보호LPS소치THP1세포손상작용상관적단백，위진일보료해이병분작용궤제제공료신선색。
Objective To observe the protein expression changes in human THP 1 stimulated by LPS ,and to discuss the possible anti-inflammatory mechanism of propofol .Methods THP1 cells were divided into control group,1 μg/ml LPS stimulation group(L group)and 1 μg/ml LPS plus 50 μmol/L propofol group(L+P group). Lysate from three THP1 groups were separated by two-dimensional electrophoresis ,then differential proteins analyzed with mass spectrometry and western Blot .Results Expressed level of nine protein spots were found significantly different in three groups with two dimensional gel electrophoresis test and image analysis .Four protein spots were identificated by mass spectrometry analysis , those were Stathmin 1, Ubiquitin-conjugating enzyme E2N, Chain A, I113t Mutant of Human Sod1 and programmed cell death 6.Conclusions Two-dimensional gel electrophoresis diagrams of THP1 cells with better repeatability and resolution rate were obtained , the protein related to anti-inflammatory function of propofol were preliminarily identificated ,so that to contribute a series of new clues for better understanding of the acted mechanism of propofol .
