CAJ | 학술논문

目的研究脂联素（APN）对脓毒症大鼠肺组织白细胞介素-6（IL-6）/信号转导及转录激活因子3（STAT3）通路的影响。方法盲肠结扎穿孔（CLP）法制作脓毒症动物模型，雄性Wistar大鼠120只，10～14周龄，体重250～300 g，随机分为对照组（ C组）、脓毒症模型组（ CLP组）和脂联素预处理组（ APN组），分别于术后2 h、6 h、12 h、24 h、48 h处死，采用酶联免疫吸附法（ELISA）检测肺组织匀浆中IL-6表达水平，半定量逆转录聚合酶链反应（ RT-PCR）法检测肺组织匀浆中IL-6 mRNA的表达水平；凝胶阻滞电泳分析技术（ EMSA）检测肺组织STAT3的DNA结合活性；同时检测肺组织髓过氧化物酶（ MPO）活性；各组剩余10只大鼠观察术后7 d的生存率，并绘制生存曲线。结果与C组比较，CLP组大鼠肺组织IL-6 mRNA及蛋白表达水平升高，STAT3的DNA结合活性表达升高，肺组织匀浆中MPO活性显著升高，死亡率升高（ P均＜0.05）；与CLP组比较，APN组大鼠肺组织IL-6 mRNA及蛋白表达水平下降，STAT3的DNA结合活性表达降低，肺组织匀浆中MPO活性显著下降，死亡率明显降低（ P均＜0.05）。结论脂联素可能通过抑制IL-6/STAT3信号转导通路，从而减轻脓毒症性肺损伤。
목적연구지련소（APN）대농독증대서폐조직백세포개소-6（IL-6）/신호전도급전록격활인자3（STAT3）통로적영향。방법맹장결찰천공（CLP）법제작농독증동물모형，웅성Wistar대서120지，10～14주령，체중250～300 g，수궤분위대조조（ C조）、농독증모형조（ CLP조）화지련소예처리조（ APN조），분별우술후2 h、6 h、12 h、24 h、48 h처사，채용매련면역흡부법（ELISA）검측폐조직균장중IL-6표체수평，반정량역전록취합매련반응（ RT-PCR）법검측폐조직균장중IL-6 mRNA적표체수평；응효조체전영분석기술（ EMSA）검측폐조직STAT3적DNA결합활성；동시검측폐조직수과양화물매（ MPO）활성；각조잉여10지대서관찰술후7 d적생존솔，병회제생존곡선。결과여C조비교，CLP조대서폐조직IL-6 mRNA급단백표체수평승고，STAT3적DNA결합활성표체승고，폐조직균장중MPO활성현저승고，사망솔승고（ P균＜0.05）；여CLP조비교，APN조대서폐조직IL-6 mRNA급단백표체수평하강，STAT3적DNA결합활성표체강저，폐조직균장중MPO활성현저하강，사망솔명현강저（ P균＜0.05）。결론지련소가능통과억제IL-6/STAT3신호전도통로，종이감경농독증성폐손상。
Objective To investigate the effect of adiponectin ( APN) on interlukin-6 and signal transducer and activator of transcription 3(STAT3)in rats with sepsis.Methods The animal model of sepsis was established by ceal ligation and puncture(CLP)in 120 Wistar male rats(weighting 250-300 g,at the age of 10-14 weeks old),which were randomly divided into 3 groups:control group ( C group ) , CLP group and adiponectin treatment group ( APN group).At 2,6,12,24,48 h after CLP,IL-6 concentration in lung homogenate was detected by enzyme linked immunosorbent assay ( ELISA ) .Lung IL-6 mRNA was detected with semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR) chain reaction(real-time RT-PCR),and the DNA-binding activity of STAT3 was analyzed by electrophoretic mobility shift assay (EMSA),while the myeloperoxidase(MPO)activity in lung tissue was determined.The mortality rate was calculated 7 d after operation and the survival curves were drawn . Results Compared with C group,gene and protein expression of IL-6 in group CLP was increased,and the DNA-binding activity of STAT3 and MPO activity in lung homogenate was raised ,as well as the mortality rate (all P<0.05 ) .Compared with CLP group , gene and protein expression of IL-6 in group APN was decreased , DNA-binding activity of STAT3 and MPO activity in lung homogenate was lower , as well as the mortality rate ( all P <0.05 ) . Conclusions APN may attenuate lung injury in CLP-induced septic rats through inhibiting the IL-6/STAT3 signaling pathway .