中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
3期
1125-1128
,共4页
付鹏%赵立军%孙鹏%刘广丽%刘兴田
付鵬%趙立軍%孫鵬%劉廣麗%劉興田
부붕%조립군%손붕%류엄려%류흥전
髓母细胞瘤%MEF2C%RNAi%Daoy细胞%形态结构
髓母細胞瘤%MEF2C%RNAi%Daoy細胞%形態結構
수모세포류%MEF2C%RNAi%Daoy세포%형태결구
Medull oblastoma%MEF2C%RNA intrference%Daoy cell%Morphological structure
目的探讨抑制人MEF2C基因后对髓母细胞瘤Daoy细胞形态结构的影响作用。方法实验分为两组：对照组和干扰组。对照组 Daoy 细胞培养感染阴性慢病毒液，干扰组 Daoy 细胞培养感染MEF2C-RNAi慢病毒液。光镜下观察培养细胞的大体形态，然后利用透射电镜观察细胞的超微结构变化情况。同时将两组细胞分别接种至裸鼠背部皮下，观察裸鼠成瘤情况以及肿瘤组织的光镜下结构和电镜下超微结构变化情况。结果干扰组Daoy细胞生长较对照组细胞生长缓慢，种植裸鼠后成瘤重量较对照组轻；光镜下显示对照组瘤组织细胞较干扰组瘤组织细胞排列密集；电镜下显示干扰组瘤组织细胞排列疏松，核固缩，凋亡小体形成。结论沉默MEF2 C基因可以引起异染色质减少、凋亡小体形成，影响细胞的形态结构，抑制肿瘤细胞生长，为其肿瘤生物治疗提供了实验依据。
目的探討抑製人MEF2C基因後對髓母細胞瘤Daoy細胞形態結構的影響作用。方法實驗分為兩組：對照組和榦擾組。對照組 Daoy 細胞培養感染陰性慢病毒液，榦擾組 Daoy 細胞培養感染MEF2C-RNAi慢病毒液。光鏡下觀察培養細胞的大體形態，然後利用透射電鏡觀察細胞的超微結構變化情況。同時將兩組細胞分彆接種至裸鼠揹部皮下，觀察裸鼠成瘤情況以及腫瘤組織的光鏡下結構和電鏡下超微結構變化情況。結果榦擾組Daoy細胞生長較對照組細胞生長緩慢，種植裸鼠後成瘤重量較對照組輕；光鏡下顯示對照組瘤組織細胞較榦擾組瘤組織細胞排列密集；電鏡下顯示榦擾組瘤組織細胞排列疏鬆，覈固縮，凋亡小體形成。結論沉默MEF2 C基因可以引起異染色質減少、凋亡小體形成，影響細胞的形態結構，抑製腫瘤細胞生長，為其腫瘤生物治療提供瞭實驗依據。
목적탐토억제인MEF2C기인후대수모세포류Daoy세포형태결구적영향작용。방법실험분위량조：대조조화간우조。대조조 Daoy 세포배양감염음성만병독액，간우조 Daoy 세포배양감염MEF2C-RNAi만병독액。광경하관찰배양세포적대체형태，연후이용투사전경관찰세포적초미결구변화정황。동시장량조세포분별접충지라서배부피하，관찰라서성류정황이급종류조직적광경하결구화전경하초미결구변화정황。결과간우조Daoy세포생장교대조조세포생장완만，충식라서후성류중량교대조조경；광경하현시대조조류조직세포교간우조류조직세포배렬밀집；전경하현시간우조류조직세포배렬소송，핵고축，조망소체형성。결론침묵MEF2 C기인가이인기이염색질감소、조망소체형성，영향세포적형태결구，억제종류세포생장，위기종류생물치료제공료실험의거。
Objective To explore the effect of silencing MEF2C gene on the morphological structure of medulloblastoma Daoy cell .Methods The experiment was divided into two groups:control group and interference group.Daoy cells of control group were infected with negative LV liquid ,and Daoy cells of interference group were infected with MEF2C-RNAi-LV liquid.Light-microscopy ( LM ) was used to observe general form of cells , and transmission electron microscopy ( TEM ) was used to observe ultrastructure changes of cells .Meanwhile, the two groups of cells were subcutaneously inoculated to the dorsum of nude mice to forn transplantation tumors , respectively ,and the morphological structures of tumor tissues were observed by LM and TEM .Results Daoy cells of interference group grew slower than cells of control group ,the weights of transplantation tumors in interference group were lighter than those in control one .The tumor tissue cells of control group had more intensive array than those of the interference group;Tumor tissue cells of interference group showed array loose ,nucleus pycnosis,apoptosis body formation under TEM.Conclusions Silencing MEF2C gene could cause heterochromatin reduce ,apoptosis body formation,and influence cell morphology , inhibit tumor cell growth .This provides experimental basis for tumor biological therapy .
