CAJ | 학술논문

目的探讨微小RNA（ miR）-122和29在乙型肝炎病毒感染相关肝病中的表达水平及其临床意义。方法应用实时荧光定量PCR方法检测肝细胞癌（ HCC ）患者（20例）、肝炎肝硬化（ LC ）患者（20例）、慢性乙型肝炎（CHB）患者（15例）、乙型肝炎病毒携带者（ASC）（20例）以及正常对照（NC）（20例）血清中miRNA-122和29的表达水平，结合临床指标进行分析。结果与NC、LC和ASC组比较，HCC和CHB组患者血清中miR-122的表达水平明显升高；与NC比较，LC组患者血清中miR-29的表达水平明显下降，差异均具有统计学意义（ P＜0.01）。相关分析显示，CHB患者血清中miR-29表达与HBV DNA呈正相关；HCC患者血清中miR-29与AFP呈负相关。结论 miR-122的表达升高与CHB的肝损伤及HCC发生有关；miR-29的表达下降与肝纤维化有关，检测其变化有助于评价乙型肝炎病毒感染者肝脏的炎症损伤和纤维化。
목적탐토미소RNA（ miR）-122화29재을형간염병독감염상관간병중적표체수평급기림상의의。방법응용실시형광정량PCR방법검측간세포암（ HCC ）환자（20례）、간염간경화（ LC ）환자（20례）、만성을형간염（CHB）환자（15례）、을형간염병독휴대자（ASC）（20례）이급정상대조（NC）（20례）혈청중miRNA-122화29적표체수평，결합림상지표진행분석。결과여NC、LC화ASC조비교，HCC화CHB조환자혈청중miR-122적표체수평명현승고；여NC비교，LC조환자혈청중miR-29적표체수평명현하강，차이균구유통계학의의（ P＜0.01）。상관분석현시，CHB환자혈청중miR-29표체여HBV DNA정정상관；HCC환자혈청중miR-29여AFP정부상관。결론 miR-122적표체승고여CHB적간손상급HCC발생유관；miR-29적표체하강여간섬유화유관，검측기변화유조우평개을형간염병독감염자간장적염증손상화섬유화。
Objective To investigate the expression and clinical significance of miR-122 and 29 in hepatitis B virus infection related liver disease .Methods The levels of miR-122 and miR-29 in serum of 20 patients with hepatocellular carcinoma ,20 patients with liver cirrhosis ,15 patients with chronic hepatitis B ,20 cases of hepatitis B virus carriers and 20 normal controls were detected by fluorescence real-time quantitative PCR method and combined with clinical correlation analysis .Results Compared with the normal control (NC),LC and ASC group,the levels of miR-122 significantly increased in the serum of patients with HCC and CHB .The levels of miR-29 in the serum of LC patients were lower than that of normal control ( P <0.01 ) .There was positive correlation between the miR-29 expression and HBV DNA in chronic hepatitis B patients .Negative correlation was found between the miR-29 and AFP in HCC patients .Conclusions The elevation of miR-122 is correlated with liver damage in chronic hepatitis B patients and the pathogenesis of liver cancer in HCC patients .The reduction of miR-29 expression is related to the incidence of liver fibrosis .Detection of miR-122 and miR-29 might help evaluate the liver inflammation injury and fibrosis.