CAJ | 학술논문

目的本研究了解天津地区汉族人群血小板膜GPⅡb HPA-3基因多态性的分布情况，探讨其与缺血性脑卒中（ IS）复发的关系。方法应用聚合酶链式反应结合限制性内切酶片段长度多态性（ PCR-RFLP）方法对全部样本进行基因分型，并抽样测序验证。结果首发病例组 ab 35.1％、aa 32.0％、bb 33.0％、a等位基因49.5％、b等位基因50.5％；对照组分别为23.7％、51.1％、25.2％、63.0％、37.0％。 b等位基因频率IS组与对照组分别是55.3％、37.0％，P＝0.000。首发组与复发组等位基因比较有显著性，其中b等位基因频率分别是50.5％、64.2％，P＝0.023。结论血小板膜GPⅡb HPA-3基因多态性与天津地区汉族人IS发生及复发相关，bb基因型可能是该人群IS的危险因素及复发高危因素，b等位基因可能是该人群IS发病及复发的危险因素。
목적본연구료해천진지구한족인군혈소판막GPⅡb HPA-3기인다태성적분포정황，탐토기여결혈성뇌졸중（ IS）복발적관계。방법응용취합매련식반응결합한제성내절매편단장도다태성（ PCR-RFLP）방법대전부양본진행기인분형，병추양측서험증。결과수발병례조 ab 35.1％、aa 32.0％、bb 33.0％、a등위기인49.5％、b등위기인50.5％；대조조분별위23.7％、51.1％、25.2％、63.0％、37.0％。 b등위기인빈솔IS조여대조조분별시55.3％、37.0％，P＝0.000。수발조여복발조등위기인비교유현저성，기중b등위기인빈솔분별시50.5％、64.2％，P＝0.023。결론혈소판막GPⅡb HPA-3기인다태성여천진지구한족인IS발생급복발상관，bb기인형가능시해인군IS적위험인소급복발고위인소，b등위기인가능시해인군IS발병급복발적위험인소。
Objective To know the GPⅡb HPA-3 polymorphism distribution of Han population in Tianjin area,and to explore the relationship between gene polymorphism of platelet membrane glycoprotein Ⅱb and recurrence of ischemic stroke in Han Nationality .Methods Using specific PCR ( PCR-RFLP ) amplification and BseGI restriction enzyome analysis ,2.5% agarose gel electrophoresis to determine genotype .Results The starting case group ab 35.1%,aa 32.0%,bb 33.0%,a allele 49.5%,b allele 50.5%;the control group ab 23.7%,aa 51.1%, bb 25.2%, a allele 63.0%, b allele 37.0%.Starting group compared with relapse group showed significant difference,in which b allele frequency were 50.5%,χ2 =5.157, P =0.023.Conclusions GPⅡb HPA-3 polymorphism may be associated with morbidity and recurrence of IS in Chinese Han population in Tianjin province . HPA-3 bb genotype is possibly risk factor for morbidity and recurrence of IS in this population .B allele may be an risk factor for onset and recurrence of IS in this population .