CAJ | 학술논문

目的研究白细胞介素10（IL-10）在伴放线放线杆菌（Aa）脂多糖（LPS）刺激兔肺泡巨噬细胞（AM ）产生炎症反应过程中的免疫调节作用及其机制。方法分离纯化AM ，100 ng/mL IL-10预孵2 h ，加入Aa-LPS刺激继续培养24 h ，流式细胞术检测AM 对荧光标记Aa（FITC-Aa）的吞噬指数（PI）情况及AM 的活性氧（ROS）水平；荧光定量PCR法检测CD14、TLR4、SRA和M HC-Ⅱ基因表达的变化；硝酸还原酶法检测AM 的NO分泌情况。结果 IL-10上调Aa-LPS诱导的AM 的PI（P＜0．05），下调Aa-LPS诱导的AM膜表面受体CD14、TLR4、M HC-Ⅱ mRNA的表达（P＜0．01），而SRA mRNA表达量升高（P＜0．05）；IL-10抑制Aa-LPS诱导的AM的ROS和NO水平（P＜0．01）。结论 IL-10可以通过上调 Aa-LPS诱导的 AM SRA 基因表达，下调CD14、TLR4、M HC-Ⅱ基因表达，并且抑制其产生ROS和NO的水平，从而调节免疫反应，限制炎症介质的大量释放，同时增强AM的吞噬作用，清除感染。
목적연구백세포개소10（IL-10）재반방선방선간균（Aa）지다당（LPS）자격토폐포거서세포（AM ）산생염증반응과정중적면역조절작용급기궤제。방법분리순화AM ，100 ng/mL IL-10예부2 h ，가입Aa-LPS자격계속배양24 h ，류식세포술검측AM 대형광표기Aa（FITC-Aa）적탄서지수（PI）정황급AM 적활성양（ROS）수평；형광정량PCR법검측CD14、TLR4、SRA화M HC-Ⅱ기인표체적변화；초산환원매법검측AM 적NO분비정황。결과 IL-10상조Aa-LPS유도적AM 적PI（P＜0．05），하조Aa-LPS유도적AM막표면수체CD14、TLR4、M HC-Ⅱ mRNA적표체（P＜0．01），이SRA mRNA표체량승고（P＜0．05）；IL-10억제Aa-LPS유도적AM적ROS화NO수평（P＜0．01）。결론 IL-10가이통과상조 Aa-LPS유도적 AM SRA 기인표체，하조CD14、TLR4、M HC-Ⅱ기인표체，병차억제기산생ROS화NO적수평，종이조절면역반응，한제염증개질적대량석방，동시증강AM적탄서작용，청제감염。
Objective To evaluate the potential immunomodulatory effects and explore the possi-ble mechanisms of interlukin 10 on activation of Actinobacillus actinomycetemcomitans(Aa)-LPS induced alveolar macrophages(AM ) . Methods Isolation and purification of AM ,AM was pre-incubated with 100 ng/mL IL-10 for 2 h and then activated by Aa-LPS for 24 h :phagocytic index (PI) was measured by flow cytometry ;RT-PCR was used to detect the expression of gene CD14 ,TLR4 ,SRA ,M HC-Ⅱ ;flow cytom-etry was used to assess ROS ;nitrate reductase assay was used to examin the amount of NO production . Results IL-10 increase the PI of Aa-LPS activated AM (P<0 .05) ,remarkably inhibited the expressions of CD14 ,TLR4 ,M HC-Ⅱ(P<0 .01) ,but enhanced the expression of SRA (P<0 .05) ,significantly sup-pressed the ROS(P<0 .01) and NO production(P<0 .01) . Conclusions IL-10 can modulate of immune response and down-regulate excessive inflammatory mediator production and enhance phagocytosis of Aa-LPS activated AM via increasing SRA ,suppressing CD14 ,TLR4 ,M HC-Ⅱexpression ,and inhibit ROS , NO production .