CAJ | 학술논문

研究了正常生长、低温胁迫和高氨氮胁迫下10个斑节对虾家系生长特性及超氧化物歧化酶(Superoxide Dismutase, SOD)、酸性磷酸酶(Acid Phosphatase, ACP)、酚氧化酶(Phenoloxidase, PO)3种免疫酶活性的变化。生长试验结果表明,10个家系特定生长率(SGR)呈现明显差异,家系077与家系017和661的ACP活性存在极显著差异,家系4612与家系017的 SOD活性存在极显著性差异,而其他家系的免疫酶活性无显著差异。斑节对虾在48h低温胁迫下, ACP和SOD活性先升高后降低, PO活性则不断降低,而对照组的3种酶活性呈无规律轻微变化。斑节对虾在48h高氨氮胁迫下, ACP活性不断升高, SOD在0-8h活性上升,8-48h活性快速降低, PO活性不断下降,而对照组的3种酶活性呈无规律轻微变化；一些家系间的3种免疫酶活性存在显著性差异,其中家系4612的3种酶活性均高于其他家系。3个试验中,家系的特定生长率与免疫酶活性均呈负相关。
연구료정상생장、저온협박화고안담협박하10개반절대하가계생장특성급초양화물기화매(Superoxide Dismutase, SOD)、산성린산매(Acid Phosphatase, ACP)、분양화매(Phenoloxidase, PO)3충면역매활성적변화。생장시험결과표명,10개가계특정생장솔(SGR)정현명현차이,가계077여가계017화661적ACP활성존재겁현저차이,가계4612여가계017적 SOD활성존재겁현저성차이,이기타가계적면역매활성무현저차이。반절대하재48h저온협박하, ACP화SOD활성선승고후강저, PO활성칙불단강저,이대조조적3충매활성정무규률경미변화。반절대하재48h고안담협박하, ACP활성불단승고, SOD재0-8h활성상승,8-48h활성쾌속강저, PO활성불단하강,이대조조적3충매활성정무규률경미변화；일사가계간적3충면역매활성존재현저성차이,기중가계4612적3충매활성균고우기타가계。3개시험중,가계적특정생장솔여면역매활성균정부상관。
The response of Superoxide Dismutase (SOD), Acid Phosphatase (ACP), and Phenoloxidase (PO) in Penaeus monodon families to normal growth, low temperature stress and high ammonia stress was studied under laboratory condition. Growth in terms of specific growth rate (SGR) was significant difference among the selected families (p<0.05). The activities of ACP were significant difference among family 077, 017 and 661. The activities of SOD were significant difference between family 4612 and family 017. When the rearing temperature decreased from 27℃to 20℃, the activities of ACP and SOD first increased and then decreased, the activities of PO reduced prolonged according to the low temperature stress, and the minor changes of control trial were not regular. Under the high ammonia stress, the activities of ACP increased prolonged, the activities of SOD increased in 0?8 h, then rapidly decreased in 8?48 h, the activities of PO reduced in 0?48 h, and the minor changes of control trial were not regular. The activities of immune enzymes of some families were significant differences, and three immune enzymes activities of family 4612 higher than that of other families under the high ammonia stress. A negative correlation was found between the specific growth rate and the activities of ACP, SOD and PO of ten families in three trials.