江苏科技大学学报(自然科学版)
江囌科技大學學報(自然科學版)
강소과기대학학보(자연과학판)
JOURNAL OF JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCE EDITION)
2013年
3期
295-299
,共5页
张磊霞%王俊%顾双双%肖维%李晶%吴福安%郭锡杰
張磊霞%王俊%顧雙雙%肖維%李晶%吳福安%郭錫傑
장뢰하%왕준%고쌍쌍%초유%리정%오복안%곽석걸
咖啡酸苯乙酯%酶促合成%离子液体%转酯化反应
咖啡痠苯乙酯%酶促閤成%離子液體%轉酯化反應
가배산분을지%매촉합성%리자액체%전지화반응
caffeic acid phenethyl ester%enzymatic synthesis%ionic liquid%transesterification raction
为提高抗肿瘤活性化合物咖啡酸苯乙酯的生物合成效率,建立离子液体中酶促转酯化合成新工艺.文中以咖啡酸甲酯和苯乙醇为原料,以脂肪酶和离子液体分别作为催化剂和反应介质,以咖啡酸苯乙酯得率为指标,考察了离子液体、脂肪酶、反应温度、底物摩尔比和酶用量等对咖啡酸苯乙酯得率的影响.结果表明:Novozym 435和[ Bmim][ Tf2 N]分别为最佳的催化剂和反应介质;当反应温度90℃、醇酯摩尔比20∶1、酶酯质量比10∶1时,转酯化反应24 h,咖啡酸苯乙酯得率达66.40%.因此,离子液体中酶促转酯化合成咖啡酸苯乙酯相比直接酯化合成法得率高,耗时短.
為提高抗腫瘤活性化閤物咖啡痠苯乙酯的生物閤成效率,建立離子液體中酶促轉酯化閤成新工藝.文中以咖啡痠甲酯和苯乙醇為原料,以脂肪酶和離子液體分彆作為催化劑和反應介質,以咖啡痠苯乙酯得率為指標,攷察瞭離子液體、脂肪酶、反應溫度、底物摩爾比和酶用量等對咖啡痠苯乙酯得率的影響.結果錶明:Novozym 435和[ Bmim][ Tf2 N]分彆為最佳的催化劑和反應介質;噹反應溫度90℃、醇酯摩爾比20∶1、酶酯質量比10∶1時,轉酯化反應24 h,咖啡痠苯乙酯得率達66.40%.因此,離子液體中酶促轉酯化閤成咖啡痠苯乙酯相比直接酯化閤成法得率高,耗時短.
위제고항종류활성화합물가배산분을지적생물합성효솔,건립리자액체중매촉전지화합성신공예.문중이가배산갑지화분을순위원료,이지방매화리자액체분별작위최화제화반응개질,이가배산분을지득솔위지표,고찰료리자액체、지방매、반응온도、저물마이비화매용량등대가배산분을지득솔적영향.결과표명:Novozym 435화[ Bmim][ Tf2 N]분별위최가적최화제화반응개질;당반응온도90℃、순지마이비20∶1、매지질량비10∶1시,전지화반응24 h,가배산분을지득솔체66.40%.인차,리자액체중매촉전지화합성가배산분을지상비직접지화합성법득솔고,모시단.
To improve the biosynthesis efficiency of caffeic acid phenethyl ester ( CAPE) with the antitumor ac-tivity , a new process for lipase-catalyzed transesterification in ionic liquid was established .In the present study , methyl caffeate ( MC) and phenylethanol ( PE) were used as substrates , lipase and ionic liquid were used as cat-alyst and reaction media , the CAPE yield was used as an evaluation index , the effects of several factors including ionic liquid, lipase, reaction temperature, substrate molar ratio and enzyme dosage on CAPE yield were as-sessed, respectively.The results indicated that Novozym 435 and [Bmim][Tf2N] were selected as the optimal catalyst and reaction media , respectively .The highest CAPE yield achieved 66.40%when the reaction after 24 h under the optimal condition as follows:temperature 90 °C, molar ratio of PE to MC 20∶1,mass ratio of No-vozym 435 to MC 10∶1.Therefore, the lipase-catalyzed synthesis of CAPE via transesterification in ionic liquid has higher yield and shorter time than direct esterification .