CAJ | 학술논문

目的制作一种新型可以缓释曲安奈德的胆道支架，观察带药支架药物释放的规律。方法将曲安奈德和聚（ DL-乳酸-羟基乙酸）共聚物（ PLGA）粉末以20％的带药浓度溶于两者的共同溶剂四氢呋喃（THF）中，并将6 Fr胆道引流管浸剪成数个50 mm段泡于上述溶剂中，10 min后取出真空烘干，常温避光保存。通过测重计算支架所载曲安奈德的质量；采用磷酸盐缓冲液（ PBS，pH 7.4）将曲安奈德稀释为系列标准液，对240 nm紫外分光峰面积进行相关分析。将曲安奈德支架放入8 ml PBS中，置于37℃的恒温摇床中持续浸泡48 h，然后换新鲜的PBS重复浸泡，直至第40天。以标准PBS液为对照组，对第1～40天留取的浸出液进行色谱分析，计算曲安奈德在浸出液中的浓度。结果称量测得平均每根支架上曲安奈德的负载量为510μg，单位面积的载药量为1.63μg/mm2。曲安奈德均能从支架表面持续释放，前5 d浓度波动在6.32～8.78μg /ml，前5 d共释放曲安奈德180.61μg，占总释放量的47.23％，之后浓度平稳波动在1.85～3.51μg/ml，第23天后开始降低，第33天的洗脱浓度为0.53μg/ml，随后即不可测得。结论用PLGA作为药物载体能成功制备曲安奈德药物胆道洗脱支架；体外研究表明该药物洗脱支架可持续稳定释放曲安奈德超过30 d。
목적제작일충신형가이완석곡안내덕적담도지가，관찰대약지가약물석방적규률。방법장곡안내덕화취（ DL-유산-간기을산）공취물（ PLGA）분말이20％적대약농도용우량자적공동용제사경부남（THF）중，병장6 Fr담도인류관침전성수개50 mm단포우상술용제중，10 min후취출진공홍간，상온피광보존。통과측중계산지가소재곡안내덕적질량；채용린산염완충액（ PBS，pH 7.4）장곡안내덕희석위계렬표준액，대240 nm자외분광봉면적진행상관분석。장곡안내덕지가방입8 ml PBS중，치우37℃적항온요상중지속침포48 h，연후환신선적PBS중복침포，직지제40천。이표준PBS액위대조조，대제1～40천류취적침출액진행색보분석，계산곡안내덕재침출액중적농도。결과칭량측득평균매근지가상곡안내덕적부재량위510μg，단위면적적재약량위1.63μg/mm2。곡안내덕균능종지가표면지속석방，전5 d농도파동재6.32～8.78μg /ml，전5 d공석방곡안내덕180.61μg，점총석방량적47.23％，지후농도평은파동재1.85～3.51μg/ml，제23천후개시강저，제33천적세탈농도위0.53μg/ml，수후즉불가측득。결론용PLGA작위약물재체능성공제비곡안내덕약물담도세탈지가；체외연구표명해약물세탈지가가지속은정석방곡안내덕초과30 d。
Objective To produce a new kind of biliary stent with Triamcinolone-eluting and observe the law of drug delivery from the stent .Methods The powder both the Triamcinolone and polylactic glycolic acid (PLGA)dissolved in the common solvent tetrahydrofuran (THF)with a drug concentration of 20%.6F biliary soaked in the above solvents .After 10 minutes,removed the vacuum drying ,and stored at room temperature .Calculated the quality of the stent contained in Triamcinolone by measuring changes in the quality .PBS buffer ( pH 7.4 ) diluted Triamcinolone for the standard solution and analyzed the ultraviolet peak area at 240 nm.Triamcinolone-eluting stents were soaked in PBS ,placed in shaker with a constant temperature of 37 ℃continuing soaked 48 h,and then soaked in fresh PBS solution until 40 d.Standard PBS solutions as the control group , the chromatographic analysis of specimens from the leaching solution for 1-40 d, calculated the concentration of Triamcinolone in the leaching solution.Results The quality of Triamcinolone contained in the stent was detected .Triamcinolone on the stent of capacity was up to 510 μg;per unit area contained in the drug amount was 1.63 μg/mm2 .Triamcinolone could be sustained-release from the stent surface.The first five days the quality release from the stent was 180.61 μg,was 47.23%of the total amount release from the stent then it fluctuated within 1.85-3.51 μg/ml.The concentration of Triamcinolone began to decrease from the 23th day,and it was 0.53 μg/ml on the 33th day,it can′t be detected in the later days.Conclusions Triamcinolone-eluting biliary stents can be successful prepared by polylactic glycolic acid as a drug carrier .In vitro study shows that the drug-eluting stents can sustainable and stable release of Triamcinolone over 30 d.