CAJ | 학술논문

目的探究环境类雌激素邻苯二甲酸二丁酯（ DBP）诱导建立小鼠隐睾模型的方法及其作用机制。方法将7～8周龄的BALB/c怀孕小鼠40只随机分为对照组（ n＝10），DBP低剂量诱导组（ n＝10）， DBP中剂量诱导组（n＝10），DBP高剂量诱导组（n＝10），在怀孕的第12天开始实验组填喂DBP，剂量分别为100 mg· kg-1· d-1、300 mg· kg -1· d-1、500 mg· kg-1· d-1，对照组填喂100 mg· kg -1· d-1的玉米油，计算新生小鼠睾丸到膀胱颈的距离与肾下极到膀胱颈距离的比值，并取睾丸组织检测NO合酶（ NOS）活性和Caspase-3蛋白水平变化。结果经DBP诱导后，小鼠睾丸到膀胱颈的距离与睾丸到肾下极距离的比值随着DBP浓度的增加显著增大（ P＜0.05）；对照组、低剂量组、中剂量组和高剂量组NOS活性分别为（0.37±0.12）nmol· min-1· g-1、（0.59±0.09）nmol· min-1· g-1、（0.98±0.11）nmol· min-1· g-1、（1.35±0.07） nmol· min-1· g-1，且随着剂量的增加 NOS 活性显著增加（ P＜0.05）；低剂量组、中剂量组和高剂量组Caspase-3蛋白活化程度分别为1.31±0.36、01.89±0.68、2.71±0.39，且随着剂量的增加Caspase-3蛋白活化程度显著加强（ P＜0.05）。结论环境类雌激素DBP能够明显诱导小鼠隐睾模型的建立，并显著改变睾丸组织的NOS活性和Caspase-3活化程度。
목적탐구배경류자격소린분이갑산이정지（ DBP）유도건립소서은고모형적방법급기작용궤제。방법장7～8주령적BALB/c부잉소서40지수궤분위대조조（ n＝10），DBP저제량유도조（ n＝10）， DBP중제량유도조（n＝10），DBP고제량유도조（n＝10），재부잉적제12천개시실험조전위DBP，제량분별위100 mg· kg-1· d-1、300 mg· kg -1· d-1、500 mg· kg-1· d-1，대조조전위100 mg· kg -1· d-1적옥미유，계산신생소서고환도방광경적거리여신하겁도방광경거리적비치，병취고환조직검측NO합매（ NOS）활성화Caspase-3단백수평변화。결과경DBP유도후，소서고환도방광경적거리여고환도신하겁거리적비치수착DBP농도적증가현저증대（ P＜0.05）；대조조、저제량조、중제량조화고제량조NOS활성분별위（0.37±0.12）nmol· min-1· g-1、（0.59±0.09）nmol· min-1· g-1、（0.98±0.11）nmol· min-1· g-1、（1.35±0.07） nmol· min-1· g-1，차수착제량적증가 NOS 활성현저증가（ P＜0.05）；저제량조、중제량조화고제량조Caspase-3단백활화정도분별위1.31±0.36、01.89±0.68、2.71±0.39，차수착제량적증가Caspase-3단백활화정도현저가강（ P＜0.05）。결론배경류자격소DBP능구명현유도소서은고모형적건립，병현저개변고환조직적NOS활성화Caspase-3활화정도。
Objective To explore the method of environmental estrogen DBP induced mice cryptorchidism model and its mechanism.Methods Forty BALB/c pregnant mice,7-8 weeks of age,were randomly divided into control group(n=10),DBP low dose induced group(n=10),DBP middle dose induced group(n=10),DBP high dose induced group ( n =10 ) .At the 12 day of pregnant , mother mice of experimental group began to be given different dose DBP,respectively for 100 mg· kg -1· d-1,300 mg· kg-1· d-1,500 mg· kg-1· d-1,control group was given corn oil of 100 mg· kg-1 · d-1.And at the 21st day,taking the fetal rat testis to detect NO synthase activity and caspase 3 protein level .Results After the DBP induced , the positive ratel of cryptorchidism mice was increased significantly ,and the ratio of the distance between mouse testicles to bladder and the lower pole of the kidney to the the bladder was increased with the increasing of the concentration of DBP .The NOS activity of control group,low dose group,middle dose group and high dose group were respectively (0.37 ±0.12 ) nmol· min-1 · g-1 , (0.59±0.09)nmol· min-1· g-1,(0.98 ±0.11)nmol· min-1· g-1,(1.35 ±0.07)nmol· min-1· g-1,and with the increase of the dose ,NOS activity increased significantly ( P<0.05 ) .The caspase-3 protein activation level in low dose group ,middle dose group and high dose group were 1.31 ±0.36 ,1.89 ±0.68 and 2.71 ±0.39 ,and with the increase of the dose , caspase-3 protein activation strengthen significantly ( P < 0.05 ) . Conclusions Environmental estrogen DBP can significantly induce the establishment of mice cryptorchidism model , and change the testicular tissue NOS activity and caspase 3 activation significantly .