中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
4期
1505-1508
,共4页
张英峰%张明明%沈洋%张洪战%胡冰
張英峰%張明明%瀋洋%張洪戰%鬍冰
장영봉%장명명%침양%장홍전%호빙
胆管肿瘤%基质金属蛋白酶7%胆汁%病例对照研究
膽管腫瘤%基質金屬蛋白酶7%膽汁%病例對照研究
담관종류%기질금속단백매7%담즙%병례대조연구
Bile duct neoplasms%Matrix metalloproteinases 7%Bile%Case-control studies
目的检测患者血清、胆汁基质金属蛋白酶7（ MMP-7）表达水平，探讨其对胆管癌的临床诊断价值。方法收集2011年12月至2012年6月东方肝胆外科医院内镜科29例胆管癌患者和20例良性狭窄患者的血清和胆汁样本。用ELISA方法检测血清、胆汁MMP-7表达水平，分析它们诊断胆管癌的敏感性、特异性及与临床参数的关系。结果血清MMP-7表达胆管癌组[（5.42±0.30）ng/ml]高于良性组[（4.28±0.19）ng/ml]，差别有统计学意义（P＝0.009）。以4.88 ng/ml（AUC 0.722，95％CI 0.580～0.865）为界值，血清MMP-7诊断胆管癌的敏感性为65.5％、特异性为80.0％。胆汁MMP-7表达胆管癌组[（10.05±0.69）ng/ml]高于良性组[（8.26±0.443）ng/ml]，差别有统计学意义（P＝0.012）；以8.32 ng/ml（AUC 0.713，95％ CI 0.564～0.862）为界值，胆汁MMP-7诊断胆管癌的敏感性为75.9％、特异性为65.0％。血清、胆汁MMP-7表达水平与临床参数无明显相关性。结论血清、胆汁MMP-7可以作为诊断胆管癌的标记物，且对鉴别胆管癌和胆道良性病变有一定的意义。
目的檢測患者血清、膽汁基質金屬蛋白酶7（ MMP-7）錶達水平，探討其對膽管癌的臨床診斷價值。方法收集2011年12月至2012年6月東方肝膽外科醫院內鏡科29例膽管癌患者和20例良性狹窄患者的血清和膽汁樣本。用ELISA方法檢測血清、膽汁MMP-7錶達水平，分析它們診斷膽管癌的敏感性、特異性及與臨床參數的關繫。結果血清MMP-7錶達膽管癌組[（5.42±0.30）ng/ml]高于良性組[（4.28±0.19）ng/ml]，差彆有統計學意義（P＝0.009）。以4.88 ng/ml（AUC 0.722，95％CI 0.580～0.865）為界值，血清MMP-7診斷膽管癌的敏感性為65.5％、特異性為80.0％。膽汁MMP-7錶達膽管癌組[（10.05±0.69）ng/ml]高于良性組[（8.26±0.443）ng/ml]，差彆有統計學意義（P＝0.012）；以8.32 ng/ml（AUC 0.713，95％ CI 0.564～0.862）為界值，膽汁MMP-7診斷膽管癌的敏感性為75.9％、特異性為65.0％。血清、膽汁MMP-7錶達水平與臨床參數無明顯相關性。結論血清、膽汁MMP-7可以作為診斷膽管癌的標記物，且對鑒彆膽管癌和膽道良性病變有一定的意義。
목적검측환자혈청、담즙기질금속단백매7（ MMP-7）표체수평，탐토기대담관암적림상진단개치。방법수집2011년12월지2012년6월동방간담외과의원내경과29례담관암환자화20례량성협착환자적혈청화담즙양본。용ELISA방법검측혈청、담즙MMP-7표체수평，분석타문진단담관암적민감성、특이성급여림상삼수적관계。결과혈청MMP-7표체담관암조[（5.42±0.30）ng/ml]고우량성조[（4.28±0.19）ng/ml]，차별유통계학의의（P＝0.009）。이4.88 ng/ml（AUC 0.722，95％CI 0.580～0.865）위계치，혈청MMP-7진단담관암적민감성위65.5％、특이성위80.0％。담즙MMP-7표체담관암조[（10.05±0.69）ng/ml]고우량성조[（8.26±0.443）ng/ml]，차별유통계학의의（P＝0.012）；이8.32 ng/ml（AUC 0.713，95％ CI 0.564～0.862）위계치，담즙MMP-7진단담관암적민감성위75.9％、특이성위65.0％。혈청、담즙MMP-7표체수평여림상삼수무명현상관성。결론혈청、담즙MMP-7가이작위진단담관암적표기물，차대감별담관암화담도량성병변유일정적의의。
Objective To determine the serum and bile MMP-7 levels in patients and investigate their diagnostic value .Methods From December 2011 to June 2012 in the Eastern Hepatobiliary Surgery Hospital Endoscopy Department 29 patients with cholangiocarcinoma ( CCA ) and 20 benign patients serum and bile samples were collected.ELISA was used to measure the serum and bile level of MMP-7.The sensitivity and specificity of MMP-7 for the diagnosis of CCA were determined ,and the relationship between its levels and clinical parameters were analyzed .Results The serum MMP-7 level in CCA group [ ( 5.42 ±0.30 ) ng/ml ] was significantly higher than benign group[(4.28 ±0.19)ng/ml,P=0.009].Using 4.88 ng/ml(AUC 0.722,95% CI 0.580-0.865)as the cutoff point ,the sensitivity and specificity of MMP-7 for diagnosis of CCA was 65.5%and 80.0%.The bile MMP-7 level in CCA group[(10.05 ±0.69)ng/ml]was significantly higher than benign group [(8.26 ±0.443)ng/ml,P=0.012 ] .Using 8.32 ng/ml( AUC 0.713 ,95%CI 0.564-0.862 ) as the cutoff point ,the sensitivity and specificity of MMP-7 for diagnosis of CCA was 75.9% and 65.0%.The serum and bile level of MMP-7 was not associated with clinical parameters.Conclusions The serum and bile MMP-7 might be useful markers of CCA ,and it may play a role in the differential diagnosis between CCA and benign disease .
