CAJ | 학술논문

目的探讨MGMT和NF-κB在脑胶质瘤中的表达及两者的相关性和MGMT基因启动子甲基化与其蛋白表达的相关性分析。方法采用免疫组化EnVision法检测MGMT和NF-κB在42例不同级别脑胶质瘤石蜡标本中的表达，运用MSP方法检测MGMT基因启动子甲基化状况。结果（1） MGMT在低级别组胶质瘤中的表达率为73.7％；在高级别组中其表达率为21.7％。（2） NF-κB在低级别组胶质瘤中的表达率为52.6％，在高级别组中的表达率为60.9％。（3）42例胶质瘤中MGMT基因启动子甲基化发生率为50％，其中MGMT蛋白阳性的胶质瘤，启动子甲基化发生率为26.3％；MGMT蛋白表达阴性的胶质瘤，启动子甲基化发生率为69.6％；MGMT蛋白表达阳性率与其基因启动子甲基化呈负相关。（4） MGMT与NF-κB两者表达无相关性。结论 MGMT蛋白表达随胶质瘤的级别增高而下降，NF-κB与MGMT与胶质瘤级别无相关性。
목적탐토MGMT화NF-κB재뇌효질류중적표체급량자적상관성화MGMT기인계동자갑기화여기단백표체적상관성분석。방법채용면역조화EnVision법검측MGMT화NF-κB재42례불동급별뇌효질류석사표본중적표체，운용MSP방법검측MGMT기인계동자갑기화상황。결과（1） MGMT재저급별조효질류중적표체솔위73.7％；재고급별조중기표체솔위21.7％。（2） NF-κB재저급별조효질류중적표체솔위52.6％，재고급별조중적표체솔위60.9％。（3）42례효질류중MGMT기인계동자갑기화발생솔위50％，기중MGMT단백양성적효질류，계동자갑기화발생솔위26.3％；MGMT단백표체음성적효질류，계동자갑기화발생솔위69.6％；MGMT단백표체양성솔여기기인계동자갑기화정부상관。（4） MGMT여NF-κB량자표체무상관성。결론 MGMT단백표체수효질류적급별증고이하강，NF-κB여MGMT여효질류급별무상관성。
Objective To investigate the expression of MGMT and NF-κB in glioma and correlation between MGMT and NF-κB,MGMT gene promoter methylation and its protein expression .Methods Immunohistochemistry assay was conducted to detect the expression of MGMT and NF-κB in gliomas;methylation specific PCR ( MSP) was used to analyze the MGMT promoter methylation in glioma .Results (1) The ratio of MGMT expression was 73.7%in the low grade group glioma;its expression was 21.7% in the high grade group .( 2 ) NF-κB expression rate was 52.6%in the low grade group glioma,expression of the high grade group was 60.9%.(3)In the 42 cases,the level of MGMT promoter methylaion was 50%;it was in 19 cases that MGMT protein expresses positively ,and among which 5 cases were methylated , so the positive expression rate was 26.3%.MGMT protein expression had negative correlation with its promoter methylation .( 4 ) There was no correlation between MGMT and NF-κB expressions . Conclusions MGMT expression decrease following the level of glioma rise and there is no correlation comparison the expression of NF-κB,and MGMT with glioma level .