中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
4期
1454-1457
,共4页
吕国庆%肖荷芳%李剑%李冠%何立锐%李亮%刘铮%肖平
呂國慶%肖荷芳%李劍%李冠%何立銳%李亮%劉錚%肖平
려국경%초하방%리검%리관%하립예%리량%류쟁%초평
结肠肿瘤%氟尿嘧啶%纳米炭%淋巴靶向化疗
結腸腫瘤%氟尿嘧啶%納米炭%淋巴靶嚮化療
결장종류%불뇨밀정%납미탄%림파파향화료
Colonic neoplasms%Fluorouracil%Carbon nanopaticles%Lymph node-targeted chemotherapy
目的探讨纳米炭为载体的淋巴靶向化疗对结肠淋巴结内转移癌细胞凋亡的影响。方法随机将2009年1月至2012年6月术前通过CT判断有或可能有淋巴转移的45例结肠癌病例随机分为：淋巴化疗组23例，术前72～48 h在纤维结肠镜下，将纳米炭-氟尿嘧啶（ CNP-5-FU）混合液多点注射于距肿瘤边缘约1.0 cm的正常结肠黏膜下。常规行结肠癌根治手术，术后整理切除标本，淋巴结分组，将取到的淋巴结分成2份，深低温冰箱保存备用。对照组22例，常规行结肠癌根治手术和标本整理，淋巴结保存同淋巴化疗组。术后对两组有淋巴转移的病例应用流式细胞分析法检测淋巴结内转移癌细胞的凋亡率，应用原位末端脱氧核苷转移酶标记法（ TUNEL法）检测淋巴结内转移癌细胞的凋亡指数。结果淋巴化疗组和对照组淋巴结内转移癌细胞凋亡率分别为（17.36±2.89）％和（1.40±0.40）％，淋巴结内转移癌细胞凋亡指数分别为（32.84±3.14）％和（13.25±2.67）％，两组比较有统计学差异（ P＜0.05）。结论载药纳米炭能够靶向性进入淋巴结内，有效诱导淋巴结内转移癌细胞的凋亡，对淋巴结转移灶具有直接治疗作用。
目的探討納米炭為載體的淋巴靶嚮化療對結腸淋巴結內轉移癌細胞凋亡的影響。方法隨機將2009年1月至2012年6月術前通過CT判斷有或可能有淋巴轉移的45例結腸癌病例隨機分為：淋巴化療組23例，術前72～48 h在纖維結腸鏡下，將納米炭-氟尿嘧啶（ CNP-5-FU）混閤液多點註射于距腫瘤邊緣約1.0 cm的正常結腸黏膜下。常規行結腸癌根治手術，術後整理切除標本，淋巴結分組，將取到的淋巴結分成2份，深低溫冰箱保存備用。對照組22例，常規行結腸癌根治手術和標本整理，淋巴結保存同淋巴化療組。術後對兩組有淋巴轉移的病例應用流式細胞分析法檢測淋巴結內轉移癌細胞的凋亡率，應用原位末耑脫氧覈苷轉移酶標記法（ TUNEL法）檢測淋巴結內轉移癌細胞的凋亡指數。結果淋巴化療組和對照組淋巴結內轉移癌細胞凋亡率分彆為（17.36±2.89）％和（1.40±0.40）％，淋巴結內轉移癌細胞凋亡指數分彆為（32.84±3.14）％和（13.25±2.67）％，兩組比較有統計學差異（ P＜0.05）。結論載藥納米炭能夠靶嚮性進入淋巴結內，有效誘導淋巴結內轉移癌細胞的凋亡，對淋巴結轉移竈具有直接治療作用。
목적탐토납미탄위재체적림파파향화료대결장림파결내전이암세포조망적영향。방법수궤장2009년1월지2012년6월술전통과CT판단유혹가능유림파전이적45례결장암병례수궤분위：림파화료조23례，술전72～48 h재섬유결장경하，장납미탄-불뇨밀정（ CNP-5-FU）혼합액다점주사우거종류변연약1.0 cm적정상결장점막하。상규행결장암근치수술，술후정리절제표본，림파결분조，장취도적림파결분성2빈，심저온빙상보존비용。대조조22례，상규행결장암근치수술화표본정리，림파결보존동림파화료조。술후대량조유림파전이적병례응용류식세포분석법검측림파결내전이암세포적조망솔，응용원위말단탈양핵감전이매표기법（ TUNEL법）검측림파결내전이암세포적조망지수。결과림파화료조화대조조림파결내전이암세포조망솔분별위（17.36±2.89）％화（1.40±0.40）％，림파결내전이암세포조망지수분별위（32.84±3.14）％화（13.25±2.67）％，량조비교유통계학차이（ P＜0.05）。결론재약납미탄능구파향성진입림파결내，유효유도림파결내전이암세포적조망，대림파결전이조구유직접치료작용。
Objective To investigate the effect of activated carbon nanoparticles targeted chemotherapy on the colon cancer metastatic cellular apoptosis in the lymph node .Methods 45 cases of colon cancer were divided into two groups randomly .Experimental group ( n=23 ):72-48 hours before radical colon cancer resection , carbon nanoparticles-fluorouracil ( CNP-5-FU) mixture was multi-point injected in normal colonic mucosa about 1.0 cm from the edge of the tumor by fibro-colonoscope .All of the lymph nodes were divided equally and restored in deep cryogenic refrigerator .Control group ( n =22 ):After routinely radical colon cancer resection , lymph nodes were restored as the Experimental group .The rate of apoptosis of metastatic cells in lymph nodes was analyzed by Flow cytometry .The cellular apoptosis index of metastatic tumor cells in lymph nodes were tested by TUNEL assay . Results The apoptosis rate of metastatic cell of Experimental group and Control group were (17.36 ±2.89 )% and (1.40 ±0.40)%,respectively.Apoptosis index of metastatic cancer cell in lymph nodes were (32.84 ±3.14)%and ( 13.25 ± 2.67 )%, respectively .The differences were significant ( both P <0.05 ) .Conclusions Drug-loading carbon nanoparticles can be imported and remained in lymph node ,effectively induce metastatic cell apoptosis in lymph node and directly treat the metastatic cancer in the lymph node .
