CAJ | 학술논문

目的研究趋化因子受体CXCR7在人早孕期母-胎界面的表达特征。方法收集人早孕期绒毛与蜕膜组织，免疫组织化学技术分析趋化因子受体CXCR7及CXCR4在母-胎界面的表达情况；分别分离培养人早孕期滋养细胞及蜕膜基质细胞，免疫细胞化学技术分析CXCR7和CXCR4在两种细胞的表达。结果人早孕期绒毛与蜕膜组织及体外分离培养的人早孕期滋养细胞、蜕膜基质细胞均可观察到特异性CXCR7和CXCR4阳性染色，但两种细胞CXCR7染色强度（平均光密度值）均略低于CXCR4，差异有统计学意义（P＜0.05）。结论人早孕期滋养细胞及蜕膜基质细胞共表达趋化因子受体CXCR7和CXCR4，提示CXCR7可能在母-胎免疫微环境形成中也起重要作用。
목적연구추화인자수체CXCR7재인조잉기모-태계면적표체특정。방법수집인조잉기융모여세막조직，면역조직화학기술분석추화인자수체CXCR7급CXCR4재모-태계면적표체정황；분별분리배양인조잉기자양세포급세막기질세포，면역세포화학기술분석CXCR7화CXCR4재량충세포적표체。결과인조잉기융모여세막조직급체외분리배양적인조잉기자양세포、세막기질세포균가관찰도특이성CXCR7화CXCR4양성염색，단량충세포CXCR7염색강도（평균광밀도치）균략저우CXCR4，차이유통계학의의（P＜0.05）。결론인조잉기자양세포급세막기질세포공표체추화인자수체CXCR7화CXCR4，제시CXCR7가능재모-태면역미배경형성중야기중요작용。
Objective To explore the expression of chemokine receptor CXCR 7 at the materno-fetal interface during human first-trimester pregnancy .Methods Human first-trimester pregnancy villous and decidual tissues were collected , and primary trophoblast cells ( TC ) and dedidual stromal cells ( DSC ) were isolated and cultured.The protein expression of CXCR7 and CXCR4 were examined by immunohistochemistry ( IHC ) and immunocytochemistry(ICC),respectively.Results IHC and ICC disclosed specific staining for both CXCR 7 and CXCR4 protein in TC and DSC ,respectively .However ,the mean density of CXCR 7 in TC and DSC was significantly lower than that of CXCR4(P<0.05).Conclusions CXCR7 and CXCR4 are co-expressed in human first-trimester pregnancy TC and DSC ,which contributes to the establishment of materno-fetal immune microenvironment .