CAJ | 학술논문

为发掘与抗白粉病基因紧密连锁的分子标记，以台烟7号为母本，NC89为父本，构建F2群体，利用烟草白粉病致病菌二孢白粉菌（Erysiphe cichoracearum DC）进行人工接种鉴定。筛选2317对SSR引物，得到61对多态稳定的引物，用其对285株F2单株进行分析，构建遗传连锁图谱。利用WinQTLCart 2.5软件对烟草白粉病抗性基因进行QTL定位。检测到1个烟草白粉病抗性基因QTL位点，与SSR引物52725紧密连锁，遗传距离为1.01cM，其加性效应为-14.38，贡献率为21.02%。
위발굴여항백분병기인긴밀련쇄적분자표기，이태연7호위모본，NC89위부본，구건F2군체，이용연초백분병치병균이포백분균（Erysiphe cichoracearum DC）진행인공접충감정。사선2317대SSR인물，득도61대다태은정적인물，용기대285주F2단주진행분석，구건유전련쇄도보。이용WinQTLCart 2.5연건대연초백분병항성기인진행QTL정위。검측도1개연초백분병항성기인QTL위점，여SSR인물52725긴밀련쇄，유전거리위1.01cM，기가성효응위-14.38，공헌솔위21.02%。
Molecular markers closely linked with resistance genes were explored with SSR technology. Population F2 was constructed from resistant variety Taiyan 7 as female parent and susceptible variety NC89 as male parent. An artificial inoculation method was adopted to test degree of the resistance to Erysiphe cichoracearum DC of their F2 family. 61 polymorphic markers obtained by screening 2317 SSR primers in 24 linkage groups were used to analyze F2 population. QTL analysis on powdery mildew resistance was conducted by WinQTLCart 2.5 software. One QTL was located on the 14th linkage group, and tightly linked with maker 52725 with genetic distance of 1.01 cM. The additive effect of QTL was-14.38 and 21.02%of phenotypic variation can be explained by QTL.