CAJ | 학술논문

为明确河南省烟草赤星病病原菌的种类，从河南省不同烟区采集烟草赤星病典型病斑，分离病原菌并且做致病性测定，对确定为病原菌的菌种按国际标准方法进一步做形态和分子鉴定。结果显示分离到的85株菌株中60株被证明是烟草赤星病病原菌，从形态学上鉴定为三个种，分别是链格孢Alternaria alternata、长柄链格孢Alternaria longipes和鸭梨链格孢Alternaria yaliinifciens。其中鸭梨链格孢Alternaria yaliinifciens引起烟草赤星病属首次报道。三个种的代表菌株的5.8S rDNA ITS基因序列间及已报道的链格孢Alternaria alternata，长柄链格孢Alternaria longipes和鸭梨链格孢Alternaria yaliinifciens的5.8S rDNA ITS区均没有较大差异，证明该基因在链格孢小孢子种间较保守，不适合区分这些链格孢种。
위명학하남성연초적성병병원균적충류，종하남성불동연구채집연초적성병전형병반，분리병원균병차주치병성측정，대학정위병원균적균충안국제표준방법진일보주형태화분자감정。결과현시분리도적85주균주중60주피증명시연초적성병병원균，종형태학상감정위삼개충，분별시련격포Alternaria alternata、장병련격포Alternaria longipes화압리련격포Alternaria yaliinifciens。기중압리련격포Alternaria yaliinifciens인기연초적성병속수차보도。삼개충적대표균주적5.8S rDNA ITS기인서렬간급이보도적련격포Alternaria alternata，장병련격포Alternaria longipes화압리련격포Alternaria yaliinifciens적5.8S rDNA ITS구균몰유교대차이，증명해기인재련격포소포자충간교보수，불괄합구분저사련격포충。
Typical disease spot specimens were collected from different areas and pathogen isolation and pathogenicity tests were carried out to determine tobacco brown spot pathogen species in Henan province. Results showed that 60 out of 85 strains isolated from disease spot were confirmed to be pathogens of tobacco brown spot by Koch's postulation. They were identified as three species of Alternaria by morphology characteristics using standard methods, i.e. Alter naria alternata, Alternaria longipes and Alternaria yaliinifciens. This is the first report of A. yaliinficiens causing tobacco brown spot. The 5.8S rDNA-ITS gene sequences of representative strains of the three species and other strains of Alternaria alternata, Alternaria longipes and Alternaria yaliinifciens show no clear difference, which proved that the gene is not suitable for distinguishing these small-spore Alternaria species.