CAJ | 학술논문

目的：利用大鼠评价酶解转变的人红细胞的安全性。方法：人红细胞与大鼠血清进行常规配血，检测二者之间的相容性；将人B型红细胞酶解后输注大鼠，流式细胞术检测人红细胞在大鼠体内的存活率。结果：人红细胞与大鼠血清不凝集，但人红细胞输注大鼠体内很快被排斥，1 h下降至50%以下，18 h被完全排斥。结论：仅依靠红细胞配血实验来选择人红细胞输血动物模型是不全面的，大鼠不适合作为酶解转变的人红细胞安全性评价的动物模型。
목적：이용대서평개매해전변적인홍세포적안전성。방법：인홍세포여대서혈청진행상규배혈，검측이자지간적상용성；장인B형홍세포매해후수주대서，류식세포술검측인홍세포재대서체내적존활솔。결과：인홍세포여대서혈청불응집，단인홍세포수주대서체내흔쾌피배척，1 h하강지50%이하，18 h피완전배척。결론：부의고홍세포배혈실험래선택인홍세포수혈동물모형시불전면적，대서불괄합작위매해전변적인홍세포안전성평개적동물모형。
Objective: To study the safety of enzymatically converted group O red blood cells(ECORBC) by rat model. Methods: The compatibility between human red blood cells(RBC) and rat was detected by blood match-ing. The B group RBC after enzymatic conversion were transfused to rats, and the survival rate of the ECORBC af-ter transfusion was detected. Results: There was no agglutination after human RBC mixed with rat blood serum. ECORBC were rejected by rats within short time, the survival rate of ECORBC decreased below 50% at 1 hour and about zero at 18 hours. Conclusion: Blood matching is not enough to select the animal model for human RBC transfusion, the rat is not suitable to appreciate the ECORBC safety.